Effects of wound dressings containing silver on skin and immune cells
Effects of wound dressings containing silver on skin and immune cells
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DOI：
10.1038/s41598-020-72249-3
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发表时间：
2020-09-16
影响因子：
4.6
通讯作者：
Velebny, Vladimir
中科院分区：
文献类型：
--
作者：
Nesporova, Kristina;Pavlik, Vojtech;Velebny, Vladimir
含银的伤口敷料在体外被证明具有细胞毒性。然而，这种细胞毒性对临床环境的推断尚不清楚。我们将不同形式的银敷料应用于体外猪皮肤，并研究了银的渗透和DNA损伤。我们评估了这些敷料的抗菌效果、对皮肤细胞的细胞毒性和诱导的免疫反应。与对照相比，所有的敷料都提高了移植皮肤中DNA损伤标记物Gamma-H(2)AX和应激相关基因的表达。这与皮肤中的银量相对应。这些敷料降低了活性，诱导了皮肤细胞的氧化应激和DNA损伤，并诱导单核细胞产生促炎IL-6。激活的中性粒细胞的氧化爆发和活性降低。不同敷料释放到培养液中的银量不同，并与体外毒性相关。然而，抗菌效率与敷料释放的银量没有很强的相关性。抗菌效率和毒性是由银的形式和敷料的结构决定的，而不仅仅是银的浓度。银敷料对体外皮肤的损伤作用凸显了在体深入研究银敷料毒性的重要性。
Wound dressings with silver have been shown to be cytotoxic in vitro. However, the extrapolation of this cytotoxicity to clinical settings is unclear. We applied dressings with various forms of silver on porcine skin ex vivo and investigated silver penetration and DNA damage. We assessed antimicrobial efficacy, cytotoxicity to skin cells, and immune response induced by the dressings. All dressings elevated the DNA damage marker gamma -H(2)AX and the expression of stress-related genes in explanted skin relative to control. This corresponded with the amount of silver in the skin. The dressings reduced viability, induced oxidative stress and DNA damage in skin cells, and induced the production of pro-inflammatory IL-6 by monocytes. The oxidative burst and viability of activated neutrophils decreased. The amount of silver released into the culture medium varied among the dressings and correlated with in vitro toxicity. However, antimicrobial efficiencies did not correlate strongly with the amount of silver released from the dressings. Antimicrobial efficiency and toxicity are driven by the form of silver and the construction of dressings and not only by the silver concentration. The damaging effects of silver dressings in ex vivo skin highlight the importance of thorough in vivo investigation of silver dressing toxicity.