Transstadial Transmission of Pythium in Bradysia impatiens and Lack of Adult Vectoring Capacity
Transstadial Transmission of Pythium in Bradysia impatiens and Lack of Adult Vectoring Capacity
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DOI：
10.1094/phyto-04-10-0117
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发表时间：
2010-12-01
期刊：
影响因子：
3.2
通讯作者：
Wraight, Stephen P.
中科院分区：
文献类型：
--
作者：
Braun, Sarah E.;Castrillo, Louela A.;Wraight, Stephen P.
Braun S E Castrillo L A Sanderson J Daughtrey M L L and wight S P， 2010凤尾迟蝇（Bradysia impatiens）中毒蝇的跨界传播及成虫传病能力的研究[J] .植物病理学100 1307 1314真菌蚊蚋传播多种产生空中传播阶段的植物病原真菌，但很少有研究表明真菌蚊蚋与包括毒蝇在内的卵菌之间可能存在相互作用真菌蚊成虫是几种常见的温室真菌属的载体，包括疣皮虫、不规则疣皮虫和最后疣皮虫。另一个目的是确定疣皮虫是否可以通过蛹体在真菌蚊幼虫的肠道中被维持，并由随后的成虫传播。在任何实验中，成虫都没有从患病植物中获取传染性的疣皮虫繁殖体并将其传播给健康植物利用实时聚合酶链反应探针检测真菌组织样品中蛇皮蚴DNA的存在，在幼虫期和蛹期检测到蛇皮蚴DNA，而在成蚊中未检测到蛇皮蚴DNA，这些结果与先前的研究结果一致，表明成蚊不太可能是蛇皮蚴的载体
Braun S E Castrillo L A Sanderson J P Daughtrey M L and Wraight S P 2010 Transstadial transmission of Pythium in Bradysia impatiens and lack of adult vectoring capacity Phytopathology 100 1307 1314Fungus gnats have been shown to transmit a variety of plant pathogenic fungi that produce aerial dispersal stages However few studies have examined potential interactions between fungus gnats and oomycetes including Pythium spp A series of laboratory experiments were conducted to determine whether fungus gnat adults are vectors of several common greenhouse Pythium spp including Pythium aphant dermatum P irregulare and P ultimum An additional objective was determine whether P aphanidermatum can be maintained transstadially in the gut of a fungus gnat larva through the pupal stadium to be transmitted by the subsequent adult Adult fungus gnats did not pick up infectious Pythium propagules from diseased plants and transmit them to healthy plants in any experiment Species specific primers and a probe for real time polymerase chain reaction were developed to detect the presence of P aphanidermatum DNA in fungus gn it tissue samples P aphanidermatum DNA was detectable in the larval and pupal stages however none was detected in adult fungus gnus These results are in agreement with previous studies that have suggested that adult fungus gnats are unlikely vectors of Pythium spp