Small-Molecule-Triggered and Light-Controlled Reversible Regulation of Enzymatic Activity
Small-Molecule-Triggered and Light-Controlled Reversible Regulation of Enzymatic Activity
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小分子触发和光控的酶活性可逆调节
DOI：
10.1021/jacs.5b11532
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发表时间：
2016
影响因子：
15
通讯作者：
Zhou Xiang
中科院分区：
文献类型：
--
作者：
Tian Tian;Song Yanyan;Wang Jiaqi;Fu Boshi;He Zhiyong;Xu Xianqun;Li Anling;Zhou Xin;Wang Shaoru;Zhou Xiang
酶活性的精细控制对于调节许多重要的细胞和有机体功能是必不可少的。蛋白质的光调控是时空调控酶产物产生和降解的重要手段。这一领域是研究人员非常感兴趣的。据我们所知，使用小分子作为光触发的分子开关，在蛋白质水平可逆地控制酶的活性尚未被研究。在本研究中，我们证明了使用小分子触发开关，这是基于偶氮苯衍生物和端粒DNA之间的分子识别的酶的光控可逆调节。该分子在365 nm紫外光和可见光交替照射下发生顺式和反式相互转化，从而引发端粒DNA的压缩和延伸。我们进一步提供了直接的证据，这种结构开关使用圆二色性研究。此外，我们的策略已成功地用于有效地控制人血浆中的凝血。
The fine control of enzyme activity is essential for the regulation of many important cellular and organismal functions. The light-regulation of proteins serves as an important method for the spatiotemporal control over the production and degradation of an enzyme product. This area is of intense interest for researchers. To the best of our knowledge, the use of small molecules as light-triggered molecular switches to reversibly control enzyme activity at the protein level has not yet been studied. In the present study, we demonstrate the light-controlled reversible regulation of the enzyme using a small-molecule-triggered switch, which is based on molecular recognition between an azobenzene derivative and telomere DNA. This molecule interconverts between thetransandcisstates under alternate 365 nm UV and visible light irradiation, which consequently triggers the compaction and extension of telomere DNA. We further provide direct evidence for this structural switch using a circular dichroism study. Furthermore, our strategy has been successfully used to effectively control blood clotting in human plasma.