Ultrastructural, histochemical, and biochemical studies of the melanin metabolism in eye and skin of pallid mice.
Ultrastructural, histochemical, and biochemical studies of the melanin metabolism in eye and skin of pallid mice.
复制标题
苍白小鼠眼睛和皮肤黑色素代谢的超微结构、组织化学和生化研究。
DOI：
10.1111/1523-1747.ep12507677
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Organisciak,DT
中科院分区：
文献类型：
--
作者：
Ito,M;Hashimoto,K;Organisciak,DT
对苍白小鼠（C57/6 J-Pa/Pa）和黑小鼠（C57/6 J −+/Pa）的毛囊和眼睛进行了超微结构、组织化学和生物化学检查，以确定色素稀释的原因。苍白小鼠毛囊和眼睛中的色素细胞比黑小鼠的成熟黑素体少。在毛囊中，苍白的黑素体转移到角质形成细胞中并聚集。在眼睛中，它们已经聚集在色素细胞内，并在酸性磷酸酶阳性溶酶体中消化。酸性磷酸酶（溶酶体酶的标志物）的活性在苍白毛囊和眼睛中显著高于黑色毛囊和眼睛.光镜和电镜水平的多巴反应表明，与黑色部分相比，每个组织中的色素细胞产生大量的多巴氧化酶.然而，L-酪氨酸-3，5 - 3 H的羟化率在苍白的眼睛中显著低于黑色的眼睛，而该速率在苍白的毛囊中显著高于黑色的毛囊。黑素体在色素细胞内的迅速消化，即自噬作用，似乎可以解释苍白小鼠眼睛活性低的原因，因此苍白小鼠的毛色和眼睛颜色变淡与多巴氧化酶活性低无关，而与黑素体的不成熟和溶酶体酶的高活性有关;这些酶似乎消化了许多这些未成熟的黑素体，并导致苍白小鼠的皮毛和眼睛颜色变淡。
The hair follicles and the eyes of pallid mice (C57/6J-Pa/Pa) and those of black mice (C57/6J−+/Pa) were examined ultrastructurally, histochemically, and biochemically to determine the cause of pigment dilution.The pigment cells in the hair follicles and the eyes of pallid mice have less mature melanosomes than those of black mice. In the hair follicles the pallid melanosomes were transferred into keratinocytes and became aggregated. In the eyes they were already aggregated within the pigment cells and were digested in acid phosphatase-positive lysosomes. The activity of acid phosphatase, a marker of lysosomal enzymes, was significantly higher in pallid hair follicles and eyes than in black hair follicles and eyes.Dopa reactions at light and electron microscopical levels indicated that the pigment cells in each tissue produced a large amount of Dopa oxidase when compared with those in each black ocunterpart. However, the rate of hydroxylation of L-tyrosine–3,5–3H was significantly lower in the pallid eyes than in black eyes, while this rate was significantly higher in pallid hair follicles than in black hair follicles. Immediate digestion of melanosomes within the pigment cells, i.e. autophagocytosis, seemed to explain the low activity in the pallid eyes.The diluted coat and eye colors of pallid mice are, therefore, not related to low Dopa oxidase activity but to immaturity of melanosomes and high activities of lysosomal enzymes; these enzymes seem to digest many of these immature melanosomes and contribute to the diluted coat and eye colors of pallid mice.
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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