DNA typing and genetic relations among populations of Kelussia odoratissima using ISSR and SRAP markers
DNA typing and genetic relations among populations of Kelussia odoratissima using ISSR and SRAP markers
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DOI：
10.1007/s00606-013-0979-3
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发表时间：
2014-06-01
影响因子：
1.9
通讯作者：
Khadivi-Khub, Abdollah
中科院分区：
文献类型：
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作者：
Hadian, Javad;Raeisi, Safiollah;Khadivi-Khub, Abdollah
香荚兰以其药用价值而闻名。它已被宣布为濒危物种。因此，研究该植物的遗传变异和保护是必要的。本研究首次采用简单重复序列（ISSR）和序列相关扩增多态性（SRAP）分子标记对77个野生紫云英个体的遗传多样性和亲缘关系进行了研究。odoratissima采自伊朗中部扎格罗斯地区的7个居群。12个ISSR引物共扩增出146条带，其中多态性条带129条（87.80%），而11个SRAP引物共扩增出86条带，其中多态性条带69条（83.30%）。结果表明，ISSR引物的多态信息含量（PIC = 0.32）、分辨率（Rp = 7.80）和标记信息量（MI = 3.48）均高于SRAP引物（PIC = 0.30、Rp = 5.61和MI = 1.88）。研究表明，利用ISSR分子标记对甘蓝型油菜的遗传变异程度进行评价比SRAP分子标记更有效。气味。在两个UPGMA聚类分析的ISSR和SRAP，在大多数情况下，来自每个群体的个人聚集在不同的群体没有明确的分离，这表明在伊朗的种质资源的高度变异。UPGMA聚类分析显示，在聚类模式的不一致，作为Mantel的测试之间的聚类图的ISSR和SRAP数据表明一个穷人适合的ISSR和SRAP数据类型（r = 0.10）。主坐标分析结果表明，前3个主坐标的变异占总变异的65.57%，将7个居群分为5个类群。这些结果对K.香子兰种质鉴定、改良和保存。
Kelussia odoratissima is well known for its medicinal importance. It has been announced as an endangered species. Thus, examining the genetic variation and conservation of this plant is necessary. In the present study, inter simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) molecular markers were employed for the first time to access the genetic diversity and relationships of 77 wild individual plants of K. odoratissima collected from seven populations in Central Zagros region of Iran. A total of 146 bands were amplified by 12 ISSR primers, of which 129 (87.80 %) were polymorphic, while 69 polymorphic bands (83.30 %) were observed among 86 bands amplified by 11 SRAP primers. Polymorphic information content (PIC = 0.32), resolving power (Rp = 7.80), and marker informativeness (MI = 3.48) generated by ISSR primers were higher than that of SRAP analysis (PIC = 0.30, Rp = 5.61, and MI = 1.88). The study indicated that ISSR were more effective than SRAP markers for assessing the degree of genetic variation of K. odoratissima. In both UPGMA dendrograms of ISSR and SRAP, in most cases, individuals from each population were clustered in various groups without clear separation, which demonstrates the high variability of this germplasm in Iran. UPGMA cluster analysis revealed inconsistencies in the clustering patterns, as the Mantel's test between the dendrograms for ISSR and SRAP data indicated a poor fit for the ISSR and SRAP data types (r = 0.10). Besides, principal coordinate analysis results showed that the first three principal coordinates account for 65.57 % of the total variation and studied seven populations were separated from each other and placed into five groups. These results have an important implication for K. odoratissima germplasm characterization, improvement, and conservation.