Modulation of immune responses by bovine β‐casein
Modulation of immune responses by bovine β‐casein
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DOI：
10.1038/icb.1996.58
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发表时间：
1996-08
影响因子：
4
通讯作者：
C. Wong;H. Seow;AI H Liu;A. Husband;G. Smithers;D. Watson
中科院分区：
文献类型：
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作者：
C. Wong;H. Seow;AI H Liu;A. Husband;G. Smithers;D. Watson
本研究报告了牛 β-酪蛋白对体外和体内免疫反应的影响。牛β-酪蛋白对绵羊中性粒细胞趋化性有抑制作用，但对中性粒细胞超氧化物产生有增强作用。为了响应有丝分裂刺激，β-酪蛋白显着增强了 T 和 B 淋巴细胞的增殖反应。虽然β-酪蛋白对绵羊血液淋巴细胞产生的IFNγ、绵羊支气管肺泡巨噬细胞产生的TNFα和MHC II类抗原表达没有显着影响，但它增强了巨噬细胞产生的IL-1β。 β-酪蛋白对牛 NK 细胞针对病毒感染细胞系的活性也没有影响。有趣的是，当肌肉注射时，β-酪蛋白被发现可以降低基质免疫刺激复合物（ISCOM）对小鼠抗卵清蛋白抗体反应的佐剂作用。综上所述，结果表明牛 β-酪蛋白在体外对反刍动物的先天性和适应性免疫反应具有选择性调节作用，而全身施用 β-酪蛋白可能对佐剂活性产生抑制作用，需要进一步研究。
The present study reports the influence of bovine β‐casein onin vitroandin vivoimmune responses. Bovine β‐casein showed an inhibitory effect on ovine neutrophil chemotaxis but had an enhancing effect on superoxide production by neutrophils. In response to mitogenic stimulation, the proliferative response of both T and B lymphocytes was significantly enhanced by β‐casein. While β‐casein had no significant effects on IFNγ production by ovine blood lymphocytes, and TNFα production and MHC Class II antigen expression by ovine bronchoalveolar macrophages, it enhanced IL‐1β production by the macrophages. β‐casein also had no influence on bovine NK cell activity against a virally‐infected cell line. Interestingly, β‐casein was found to reduce the adjuvant effect of matrix immune stimulating complexes (ISCOM) on anti‐ovalbumin antibody response in mice when given intramuscularly. Taken together, the results suggest that bovine β‐casein had selective modulating effectsin vitroon both innate and adaptive immune responses in ruminants, whereas systemic administration of β‐casein, that might have a depressive effect on adjuvant activity, requires further study.