STRUCTURE OF AMPHOTERICIN-B AGGREGATES AS REVEALED BY UV AND CD SPECTROSCOPIES
STRUCTURE OF AMPHOTERICIN-B AGGREGATES AS REVEALED BY UV AND CD SPECTROSCOPIES
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DOI：
10.1002/bip.1981.360200802
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
DUPONT, G
中科院分区：
文献类型：
--
作者：
ERNST, C;GRANGE, J;DUPONT, G
庚烯大环内酯阿氏菌素B的水溶液和水醇溶液在CD [圆二色性]和吸收光谱中在π * 范围内显示出强的和可变的信号。. rarw. . π。过渡光谱变化的解释提出了基于2种形式的分子间组织之间的平衡：聚集的一个（A）与强激子相互作用和非聚集的一个（B），其光谱类似于那些线性共轭多烯在真正的解决方案与发达的振动结构。通过A型和B型光谱的线性组合拟合中间光谱。A-型的聚集体的2级组织被提出：几个分子的紧密包装，这是吸收最大值的hypochromic移位的起源;和聚集体内部的前面的小单元之间的相互作用，这是光谱上表示的强烈的CD偶联。
The aqueous and hydroalcoholic solutions of the heptenic macrolide amphotericin B display strong and variable signals in CD [circular dichroism] and absorption spectroscopies in the range of the .pi.* .rarw. .pi. transition. An interpretation of the spectroscopic changes is proposed based on the equilibrium between 2 forms of the intermolecular organization: the aggregated one (A) with strong excitonic interaction and the nonaggregative one (B) whose spectra are like those of linear conjugated polyenes in true solution with a well-developed vibrational structure. The intermediate spectra are fitted by linear combination of the A- and B-form spectra. A 2-level organization of the aggregates is proposed for the A-form: a close packing of few molecules, which is the origin of the absorption maxima hypsochromic shift; and interaction between the preceding small units inside the aggregates, which is spectroscopically expressed by the intense CD couplet.