A comparison of dopaminergic cells from the human NTera2/D1 cell line transplanted into the hemiparkinsonian rat.
A comparison of dopaminergic cells from the human NTera2/D1 cell line transplanted into the hemiparkinsonian rat.
复制标题
来自移植到偏侧帕金森病大鼠中的人 NTera2/D1 细胞系的多巴胺能细胞的比较。
DOI：
10.1016/j.lfs.2007.05.025
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发表时间：
2007
期刊：
影响因子：
6.1
通讯作者：
Willing,AE
中科院分区：
文献类型：
--
作者：
Newcomb,JD;Janowski,M;Zigova,T;Saporta,S;McGrogan,MP;Sanberg,PR;Willing,AE
来源于NTera2/D1细胞系的人NT细胞表达多巴胺能表型，使它们成为向帕金森患者枯竭的纹状体供应多巴胺的有吸引力的载体。在体外，HNT神经元表达酪氨酸羟化酶(TH)，这取决于它们暴露于维甲酸的时间长短。这项研究比较了两类HNT神经元，MI-HNT和DA-HNT，它们的TH+细胞产量都很高。将MI-HNT和DA-HNT神经元移植到6-OHDA偏侧帕金森病大鼠的纹状体内。观察大鼠旋转行为的改善情况，并用免疫组织化学方法鉴定存活的HNT和TH+HNT神经元。结果表明，MI-HNT和DA-HNT神经元均能在纹状体存活，但在体内均不能维持其多巴胺能表型。与HNT细胞替代联合使用的其他策略可能需要增强和维持移植细胞中的多巴胺表达。
Human NT cells derived from the NTera2/D1 cell line express a dopaminergic phenotype making them an attractive vehicle to supply dopamine to the depleted striatum of the Parkinsonian patient. In vitro, hNT neurons express tyrosine hydroxylase (TH), depending on the length of time they are exposed to retinoic acid. This study compared two populations of hNT neurons that exhibit a high yield of TH+ cells, MI-hNT and DA-hNT. The MI-hNT and DA-hNT neurons were intrastriatally transplanted into the 6-OHDA hemiparkinsonian rat. Amelioration in rotational behavior was measured and immunohistochemistry was performed to identify surviving hNT and TH+ hNT neurons. Results indicated that both MI-hNT and DA-hNT neurons can survive in the striatum, however, neither maintained their dopaminergic phenotype in vivo. Other strategies used in conjunction with hNT cell replacement are likely needed to enhance and maintain the dopamine expression in the grafted cells.