Diversity and history of the long-chain acyl-CoA synthetase (Acsl) gene family in vertebrates.
Diversity and history of the long-chain acyl-CoA synthetase (Acsl) gene family in vertebrates.
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DOI：
10.1186/1471-2148-13-271
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发表时间：
2013-12-12
影响因子：
3.4
通讯作者：
Castro LF
中科院分区：
文献类型：
--
作者：
Lopes-Marques M;Cunha I;Reis-Henriques MA;Santos MM;Castro LF
脂肪酸是脂质分子的重要组成部分，参与基本的生理过程。它们经历活化成其相应的CoA酯，用于氧化或酯化成复合脂质（例如甘油三酯、磷脂和胆固醇酯），这是由酰基CoA脱氢酶（ACS）进行的过程。在这里，我们分析了进化的基因家族编码的长链酰基辅酶A合成酶（Acsl）在脊椎动物。通过遗传学和比较基因组学的方法，我们发现基因组重复（2R）产生了现存脊椎动物谱系中Acsl基因的多样性。在脊椎动物祖先中，两个独立的基因起源于当前的Acsl1/5/6和Acsl3/4基因家族，硬骨鱼类中的额外基因重复是硬骨鱼类特异性第三轮基因组重复（3R）的结果。此外，Acsl家族成员的多样性比预期的更广泛。我们的策略发现了一种新的未表征的Acsl样基因，发现在硬骨鱼，斑点雀鳝，腔棘鱼，可能七鳃鳗，我们指定Acsl2。Acsl2硬骨鱼基因座的详细分析强烈支持的结论，它对应于保留的2R parabolic，失去了四足动物。我们在这里提供的第一个进化分析的Acsl基因家族在脊椎动物中，显示特定的贡献，2R/3R的多样性，这个基因家族。我们还发现，ACSL酶分为两组之前，至少出现后口动物。我们的研究表明，基因组复制显着有助于脊椎动物脂肪酸活化代谢的阐述。
Fatty acids, a considerable fraction of lipid molecules, participate in fundamental physiological processes. They undergo activation into their corresponding CoA esters for oxidation or esterification into complex lipids (e.g. triglycerides, phospholipids and cholesterol esters), a process that is carried out by acyl-CoA synthases (ACS). Here we analyze the evolution of the gene family encoding for the long-chain acyl-CoA synthetases (Acsl) in vertebrates. By means of phylogenetics and comparative genomics we show that genome duplications (2R) generated the diversity of Acsl genes in extant vertebrate lineages. In the vertebrate ancestor two separate genes originated the current Acsl1/5/6 and the Acsl3/4 gene families, and the extra gene duplicates in teleosts are a consequence of the teleost specific third round of genome duplication (3R). Moreover, the diversity of Acsl family members is broader than anticipated. Our strategy uncovered a novel uncharacterized Acsl-like gene found in teleosts, spotted gar, coelacanth and possibly lamprey, which we designate Acsl2. The detailed analysis of the Acsl2 teleost gene locus strongly supports the conclusion that it corresponds to a retained 2R paralogue, lost in tetrapods. We provide here the first evolutionary analysis of the Acsl gene family in vertebrates, showing the specific contribution of 2R/3R to the diversity of this gene family. We find also that the division of ACSL enzymes into two groups predates at least the emergence of deuterostomes. Our study indicates that genome duplications significantly contributed to the elaboration of fatty acid activation metabolism in vertebrates.
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DOI：
10.1093/bioinformatics/btq224
发表时间：
2010-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Katoh K;Toh H
通讯作者：
Toh H
影响因子：
2.9
作者：
Castro, LFC;Hoiianda, PWH
通讯作者：
Hoiianda, PWH
影响因子：
7.7
作者：
Morino, Katsutaro;Petersen, Kitt Falk;Shulman, Gerald I.
通讯作者：
Shulman, Gerald I.
影响因子：
10.7
作者：
Mulley JF;Holland PW
通讯作者：
Holland PW
影响因子：
3
作者：
Morais, Sofia;Monroig, Oscar;Tocher, Douglas R.
通讯作者：
Tocher, Douglas R.