Effects of paraquat on canine bronchoalveolar lavage fluid.
Effects of paraquat on canine bronchoalveolar lavage fluid.
复制标题
百草枯对犬支气管肺泡灌洗液的影响。
DOI：
10.1016/0041-008x(89)90226-3
复制
发表时间：
1989
影响因子：
3.8
通讯作者：
Pond,SM
中科院分区：
文献类型：
--
作者：
Hampson,EC;Eyles,DW;Pond,SM
支气管肺泡灌洗(BAL)可恢复下呼吸道上皮肺液。在这项研究中，我们用BAL检测了Beagle犬静脉滴注10 mg/kg体重百草枯后的早期肺损伤。11只犬分别于静脉滴注百草枯(n=8)或生理盐水(n=3)前60h和后34h进行两次支气管肺泡灌洗。实验分为三组：(1)单纯百草枯(n=4)；(2)百草枯+血液灌流(n=4)；(3)单纯血液灌流(n=3)。由于血液灌流是一种治疗百草枯中毒的方法，可以独立于百草枯对BAL产生影响，因此我们评估了该程序与百草枯单独应用以及与百草枯联合应用对BAL液的影响。我们检查了BAL液中的细胞学、蛋白质、酶和谷胱甘肽，并表达了每毫升吸入灌洗液的所有结果。血液灌流不改变BAL液。相比之下，研究犬在服用百草枯34小时后，细胞总数、肺泡巨噬细胞和中性粒细胞计数以及总蛋白、白蛋白、ACE、LDH和ALP浓度增加。犬的支气管肺泡灌洗为检测早期百草枯所致肺损伤提供了一种极好的工具。同样的技术也可以用于其他类型的肺部疾病和损伤的顺序监测。
Bronchoalveolar lavage (BAL) recovers the epithelial lung fluid of the lower respiratory tract. In this study, we have used BAL to detect early pulmonary injury in beagle dogs following an intravenous infusion of 10 mg paraquat dichloride/kg bodyweight. Bronchoalveolar lavage was performed twice in 11 dogs, 60 hr before and 34 hr after an intravenous infusion of paraquat dichloride (n = 8) or saline (n = 3). The dogs were studied in three groups: (1) paraquat only (n = 4); (2) paraquat plus hemoperfusion (n = 4); and (3) hemoperfusion only (n = 3). Because hemoperfusion, a treatment used for paraquat poisoning, could have effects on BAL independent of paraquat, we evaluated the effects on BAL fluid of this procedure performed separately from and together with administration of paraquat. We examined cytology, proteins, enzymes, and glutathione in the BAL fluid and expressed all results per milliliter of aspirated lavage fluid. Hemoperfusion did not alter the BAL fluid. In contrast, in dogs studied 34 hr after administration of paraquat, total cell counts, alveolar macrophage and neutrophil counts, and concentrations of total protein, albumin, ACE, LDH, and ALP were increased. Bronchoalveolar lavage in the dog provides an excellent tool with which to detect early paraquat-induced pulmonary injury. The same technique could be useful for sequential monitoring of other types of pulmonary disease and injury.