Gene therapy using bacterial vectors
Gene therapy using bacterial vectors
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DOI：
10.2741/4473
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发表时间：
2017-01-01
影响因子：
3.1
通讯作者：
Gardlik, Roman
中科院分区：
文献类型：
--
作者：
Celec, Peter;Gardlik, Roman
细菌可以通过两种策略用于基因治疗——要么使用细菌-细菌感染转染真核宿主细胞，要么使用不改变宿主基因组，但使用可以从外部控制或阻止的原核表达系统的替代基因治疗。虽然细菌感染是基因替代和DNA疫苗接种的最佳选择，但替代基因治疗适用于蛋白质的原位传递和细胞内杆菌病的治疗。细菌介导的基因治疗的一种特殊形式是跨王国RNA干扰。本文综述了细菌载体在生物医学研究中的主要应用及其优势和存在的问题。尽管发表了大量的实验，特别是在治疗实体肿瘤和肠道感染方面，但临床的进展滞后，需要在这些方法的安全性甚至效率方面进行重大改进。
Bacteria can be used for gene therapy via two strategies - either by transfection of eukaryotic host cells using bacteria - bactofection or by alternative gene therapy that does not alter the host genome, but uses the prokaryotic expression system that can be controlled or stopped from outside. While bactofection is optimal for gene substitution and DNA vaccination, alternative gene therapy is suitable for in situ delivery of proteins and treatment with intracellular bactochondria. A specific form of bacteria-mediated gene therapy is the transkingdom RNA interference. In this review advantages and issues related to bacterial vectors as well as the major applications in biomedical research are summarized. Despite numerous published experiments, especially in the treatment of solid tumors and gut infections, the progress in the clinics lags behind and major improvements in the safety and even more in the efficiency of these approaches are needed.