Mechanisms of stiffening and strengthening in media-equivalents fabricated using glycation
Mechanisms of stiffening and strengthening in media-equivalents fabricated using glycation
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DOI：
10.1115/1.429652
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发表时间：
2000-06-01
影响因子：
1.7
通讯作者：
Tranquillo, RT
中科院分区：
文献类型：
--
作者：
Girton, TS;Oegema, TR;Tranquillo, RT
我们最近报道，糖化可用于增加介质等效物 (ME) 的圆周拉伸刚度和极限拉伸强度，并增加其对胶原蛋白分解降解的抵抗力，所有这些都不会损失细胞活力 (Girton 等人，1999)。糖化 ME 是通过将高传代成年主动脉 SMC 包埋在由胃蛋白酶消化的牛真皮胶原制成的胶原凝胶中制成的，并在添加了 30 mM 核糖的完全培养基中孵育长达 10 周。我们报告的实验表明，由于 SMC 施加的牵引力导致的 ME 压实以及随后的胶原纤维排列对于实现糖基化介导的硬化和强化是必要的，但这些细胞合成细胞外基质成分可能贡献很小，甚至在培养基中添加了 50 微克/毫升抗坏血酸。这些糖化 ME 表现出与动脉相似的顺应性，但抗拉强度较低，破裂强度也较低。用低通道而不是高通道成年大鼠主动脉 SMC 制造的 ME 具有几乎十倍的拉伸强度，这表明除了已经获得的结构重塑（即圆周排列）之外，替代的 SMC 来源和生物聚合物凝胶可以通过植入前的成分重塑产生足够的强度。
We have recently reported that glycation can be exploited to increase the circumferential tensile stiffness and ultimate tensile strength of media-equivalents (MEs) and increase their resistance to collagenolytic degradation, all without loss of cell viability (Girton et at., 1999). The glycated MEs were fabricated by entrapping high passage adult mt aorta SMCs in collagen gel made from pepsin-digested bovine dermal collagen, and incubated for up to 10 weeks in complete medium with 30 mM ribose added. We report hei-e on experiments showing that ME compaction due to traction exerted by the SMCs with consequent alignment of collagen fibrils was necessary to realize the glycation-mediated stiffening and strengthening, but that synthesis of extracellular matrix constituents by these cells likely contributed little, even wizen 50 mug/ml ascorbate was added to the medium. These glycated MEs exhibited a compliance similar to arteries, brit possessed less tensile strength and much less burst strength. MEs fabricated with low rather than high passage adult rat aorta SMCs possessed almost ten times greater tensile strength, suggesting that alternative SMCs sources and biopolymer gels may yield sufficient strength by compositional remodeling prior to implantation in addition to the structural remodeling (ie., circumferential alignment) already obtained.