Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes
Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes
复制标题
葡萄糖代谢途径对猪卵母细胞核和细胞质成熟的影响
DOI：
10.1038/s41598-020-59709-6
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发表时间：
2020-02-17
影响因子：
4.6
通讯作者：
Tan, Jing-He
中科院分区：
文献类型：
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作者：
Wen, Jing;Wang, Guo-Liang;Tan, Jing-He
猪体外成熟卵母细胞的发育能力与其在体对照相比仍较低。葡萄糖代谢（GM）对卵母细胞核成熟的影响研究较多，但对胞质成熟的影响研究较少。以往的研究不能区分卵丘细胞（CC）和去卵丘卵母细胞（DO）的GM是否支持卵母细胞的成熟。此外，卵母细胞GM的物种差异在很大程度上是未知的。我们的目的是通过使用酶活性抑制剂，RNAi基因沉默和特殊培养基来解决这些问题，当GM被抑制时，这些培养基可以支持细胞核而不是细胞质成熟。结果表明，转基因猪卵母细胞通过磷酸戊糖途径和糖酵解途径促进卵母细胞成熟。在猪DO和CC中，丙酮酸和乳酸都通过单羧酸转运体转运到猪DO中，丙酮酸通过线粒体丙酮酸载体进一步转运到线粒体中。在猪DO和CC中，丙酮酸和乳酸分别通过线粒体电子传递和LDH催化氧化为丙酮酸而被利用。猪和小鼠DO在葡萄糖、丙酮酸和乳酸利用的CC依赖性方面不同。虽然小鼠DO不能，但猪DO可以使用乳酸衍生的丙酮酸。
The developmental competence of IVM porcine oocytes is still low compared with that in theirin vivocounterparts. Although many studies reported effects of glucose metabolism (GM) on oocyte nuclear maturation, few reported on cytoplasmic maturation. Previous studies could not differentiate whether GM of cumulus cells (CCs) or that of cumulus-denuded oocytes (DOs) supported oocyte maturation. Furthermore, species differences in oocyte GM are largely unknown. Our aim was to address these issues by using enzyme activity inhibitors, RNAi gene silencing and special media that could support nuclear but not cytoplasmic maturation when GM was inhibited. The results showed that GM in CCs promoted pig oocyte maturation by releasing metabolites from both pentose phosphate pathway and glycolysis. Both pyruvate and lactate were transferred into pig DOs by monocarboxylate transporter and pyruvate was further delivered into mitochondria by mitochondrial pyruvate carrier in both pig DOs and CCs. In both pig DOs and CCs, pyruvate and lactate were utilized through mitochondrial electron transport and LDH-catalyzed oxidation to pyruvate, respectively. Pig and mouse DOs differed in their CC dependency for glucose, pyruvate and lactate utilization. While mouse DOs could not, pig DOs could use the lactate-derived pyruvate.