Structural characterization of teichoic acids from Lactobacillus brevis
Structural characterization of teichoic acids from Lactobacillus brevis
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DOI：
10.1016/j.carres.2009.12.007
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发表时间：
2010-02-26
影响因子：
3.1
通讯作者：
Holst, Otto
中科院分区：
文献类型：
--
作者：
Carballo, Patricia M. Sanchez;Vilen, Heikki;Holst, Otto
磷壁酸是革兰氏阳性菌细胞壁的主要成分。使用一维和二维核磁共振实验以及化学方法对从短乳杆菌中分离出的脂磷壁酸和磷壁酸进行了结构表征。成分分析表明存在大量甘油、葡萄糖和丙氨酸。在 LTA 的情况下，还检测到了十八碳烯酸。基本的LTA/WTA结构被确定为在甘油残基的C-2处被D-Ala或α-D-Glc非化学计量取代的1,3-聚(磷酸甘油)。在 LTA 的情况下，可以检测到较高量的 Ala，并且还可以观察到 Glc 位置 C-6 处的部分丙氨酰化。 (C) 2009 Elsevier Ltd. 保留所有权利。
Teichoic acids are a major constituent of the cell wall of Gram-positive bacteria. Structural characterization of lipoteichoic and teichoic acids isolated from Lactobacillus brevis was undertaken using 1D and 2D NMR experiments as well as chemical methodology. Compositional analysis indicated the presence of high amounts of glycerol, glucose, and alanine. In the case of LTA octadecenoic acid was also detected. The basic LTA/WTA structure was identified as 1,3-poly(glycerol phosphate) nonstoichiometrically substituted at C-2 of the glycerol residues with D-Ala or alpha-D-Glc. In the case of LTA a higher amount of Ala could be detected and partial alanylation at position C-6 of the Glc could also be observed. (C) 2009 Elsevier Ltd. All rights reserved.