An assessment of morphogenetic fluctuation during reproductive phase change in Arabidopsis
An assessment of morphogenetic fluctuation during reproductive phase change in Arabidopsis
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DOI：
10.1093/aob/mcr039
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发表时间：
2011-05-01
期刊：
影响因子：
4.2
通讯作者：
Albertini, Catherine
中科院分区：
文献类型：
--
作者：
Pouteau, Sylvie;Albertini, Catherine
拟南芥（Arabidopsis thaliana）的生殖阶段变化的特征在于植物体身份的两个转变，第一伸长节间的分化（抽薹转变）和第一朵花的分化（成花转变）。这些过渡的动态评估寻求通过检查相应的phytomer身份changes.Methods的精度第一伸长节间的长度和频率的嵌合花序结构，例如。G.在Wassilewskija（Ws）材料和47个早花突变体中，在宽范围的光周期下测量了不被叶包着的paraclades（无叶/paraclades）和被苞片包着的花（苞片/花）。光周期扰动的影响适用于Ws植物在不同的时间的发展也evaluated.Key结果在Ws，这两种类型的字符是显着恒定的光周期，尽管高度的个体间变异。苞片/花通常不会在Ws中产生，但在暗示增强光信号的条件下观察到它们，例如。G.在对连续光扰动的响应中以及在下胚轴伸长减少的突变体中。相比之下，无叶/paraclades通常存在于约。20%的Ws植物，并且在表明光信号减少的条件下，它们的频率增加，例如。G.在长日照反应的变异体中。第一伸长节间的长度是不相关的茎伸长的速率和生殖phase changes.Conclusions苞片/花和无叶/paraclades的调节花的过渡，反映了不同的动态进展到开花的相反的效果。相反，第一伸长节间的长度仅与生殖阶段变化的调节间接相关，并且主要取决于全局形态发生约束。本文提出，形态发生的变异性可以用来确定发展的关键阶段和发育模式的渠化特征。
Background and Aims Reproductive phase change in Arabidopsis thaliana is characterized by two transitions in phytomer identity, the differentiation of the first elongate internode (bolting transition) and of the first flower (floral transition). An evaluation of the dynamics of these transitions was sought by examining the precision of the corresponding phytomer identity changes.Methods The length of the first elongate internode and the frequency of chimeric inflorescence structures, e. g. paraclades not subtended by a leaf (no-leaf/paraclades) and flowers subtended by a bract (bract/flowers), were measured in the Wassilewskija (Ws) accession and 47 early flowering mutants under a wide range of photo-periods. The impact of photoperiodic perturbations applied to Ws plants at different times of development was also evaluated.Key Results In Ws, both types of characters were remarkably constant across photoperiods in spite of a high degree of interindividual variability. Bract/flowers were not normally produced in Ws, but they were observed in conditions that suggest enhanced light signalling, e. g. in response to continuous light perturbations and in mutants with reduced hypocotyl elongation. In contrast, no-leaf/paraclades were normally present in approx. 20% of Ws plants, and their frequency was increased in conditions that suggest reduced light signalling, e. g. in mutants with altered specification of long-day responses. The length of the first elongate internode was unrelated to the rate of stem elongation and to the regulation of reproductive phase change.Conclusions Bract/flowers and no-leaf/paraclades corresponded to opposite effects on the floral transition that reflected different dynamics of progression to flowering. In contrast, the length of the first elongate internode was only indirectly related to the regulation of reproductive phase change and was mainly dependent on global morphogenetic constraints. This paper proposes that morphogenetic variability could be used to identify critical phases of development and characterize the canalization of developmental patterns.