Discrete DNA sites regulate global distribution of meiotic recombination.
Discrete DNA sites regulate global distribution of meiotic recombination.
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离散 DNA 位点调节减数分裂重组的全局分布。
DOI：
10.1016/j.tig.2010.02.003
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Davidson,MariK
中科院分区：
文献类型：
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作者：
Wahls,WayneP;Davidson,MariK
同源重组在减数分裂中被诱导到高水平，由Spo11催化的DNA双链断裂（DSB）启动，并聚集在调节其在基因组中定位的热点处。在减数分裂中，染色体的正确分离需要染色体分离，而其频率或定位的缺陷会导致染色体错误分离，从而导致先天性出生缺陷，如唐氏综合征。因此，阐明减数分裂重组是如何定位的基本和生物医学的兴趣。我们整合了该领域的历史和当代进展，再加上对已发表的基因组范围内重组分布的微阵列数据的重新分析，支持了这种调控的统一模型。我们认为，离散的DNA序列基序的位置和调节基本上所有的重组基因组，在很大程度上相同的方式，DNA位点的位置和调节转录。此外，我们说明了使用重叠机制的转录和减数分裂重组的调节。结合的转录因子诱导组蛋白修饰，将重组定位在热点。
Homologous recombination is induced to high levels in meiosis, is initiated by Spo11-catalyzed DNA double-strand breaks (DSBs) and is clustered at hotspots that regulate its positioning in the genome. Recombination is required for proper chromosome segregation in meiosis and defects in its frequency or positioning cause chromosome mis-segregation and, consequently, congenital birth defects such as Down's syndrome. Therefore, elucidating how meiotic recombination is positioned is of fundamental and biomedical interest. Our integration of historical and contemporary advances in the field, plus the re-analysis of published microarray data on the genome-wide distribution of recombination supports a unifying model for such regulation. We posit that discrete DNA sequence motifs position and regulate essentially all recombination across the genome, in much the same way that DNA sites position and regulate transcription. Moreover, we illustrate the use of overlapping mechanisms for the regulation of transcription and meiotic recombination. Bound transcription factors induce histone modifications that position recombination at hotspots.