Snail family genes are required for left-right asymmetry determination, but not neural crest formation, in mice
Snail family genes are required for left-right asymmetry determination, but not neural crest formation, in mice
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DOI：
10.1073/pnas.0602234103
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发表时间：
2006-07-05
影响因子：
11.1
通讯作者：
Gridley, Thomas
中科院分区：
文献类型：
--
作者：
Murray, Stephen A.;Gridley, Thomas
蜗牛家族基因编码锌指转录抑制因子，这些转录抑制因子是脊椎动物上皮-间质转化的关键调节因子，包括产生中胚层和神经嵴的转化。在这里，我们表明，与在青蛙和鸟类胚胎中观察到的相反，在小鼠神经嵴的形成和分层中不需要蜗牛家族基因Snail（蜗牛）和Slug（蜗牛）。然而，条件失活蜗牛功能的胚胎表现出左右不对称决定的缺陷。这项工作表明，尽管蜗牛家族基因功能的某些方面，如左右不对称决定的作用，似乎在进化上是保守的，但它们在神经嵴细胞形成和分层中的作用却不是。
Snail family genes encode zinc finger transcriptional repressors that are key regulators of epithelial-mesenchymal transitions in vertebrates, including the transitions that generate the mesoderm and neural crest. Here, we show that, contrary to observations in frog and avian embryos, the Snail family genes Snail (Snail) and Slug (Snail) are not required for formation and delamination of the neural crest in mice. However, embryos with conditional inactivation of Snail function exhibit defects in left-right asymmetry determination. This work demonstrates that although some aspects of Snail family gene function, such as a role in left-right asymmetry determination, appear to be evolutionarily conserved, their role in neural crest cell formation and delamination is not.