Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples
Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples
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DOI：
10.1038/nmeth.1211
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发表时间：
2008-06-01
期刊：
影响因子：
48
通讯作者：
Bewersdorf, Joerg
中科院分区：
文献类型：
--
作者：
Juette, Manuel F.;Gould, Travis J.;Bewersdorf, Joerg
需要在三维（3D）超分辨率下成像与整个细胞一样厚的体积，以揭示细胞组织的未知特征。我们报告一个光学显微镜，产生的图像与几何不变的30 × 30 × 75纳米的分辨率超过几微米的深度。这种方法，命名为双平面（BP）FPALM，结合了双平面检测方案与荧光光活化定位显微镜（FPALM），使3D亚衍射分辨率，而不影响速度或灵敏度。
Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.