Quantification of butyryl CoA:acetate CoA-transferase genes reveals different butyrate production capacity in individuals according to diet and age
Quantification of butyryl CoA:acetate CoA-transferase genes reveals different butyrate production capacity in individuals according to diet and age
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DOI：
10.1111/j.1574-6968.2010.02197.x
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发表时间：
2011-03-01
影响因子：
2.1
通讯作者：
Haslberger, Alexander G.
中科院分区：
文献类型：
--
作者：
Hippe, Berit;Zwielehner, Jutta;Haslberger, Alexander G.
胃肠道微生物群产生短链脂肪酸，尤其是丁酸酯，影响结肠健康、免疫功能和表观遗传调控。为了评估营养和老化对丁酸酯生产的影响，分析了丁酸酯主要产生菌lV和XlVa梭菌群的丁基辅酶a：乙酸辅酶a转移酶基因和种群迁移。对年轻健康杂食者（24 +/- 2.5岁）、素食者（26 +/- 5岁）和老年杂食者（86 +/- 8岁）的粪便样本进行了评价。饮食和生活方式通过问卷调查进行评估。老年人的丁基辅酶a：醋酸辅酶a转移酶基因拷贝数明显少于年轻杂食动物（P=0.014），而素食者的拷贝数最高（P=0.048）。与玫瑰菌/直肠真杆菌相关的丁基辅酶a：乙酸辅酶a转移酶基因变异融化曲线的热变性在素食者中比在老年人中变化更大。与老年组相比，素食者（P=0.049）和杂食者（P < 0.01）中Clostridium cluster XIVa的丰度更高。老年人胃肠道微生物群的特点是丁酸盐生产能力下降，反映出退行性疾病的风险增加。这些结果表明，丁基辅酶a：醋酸辅酶a转移酶基因是胃肠道微生物群功能的一个有价值的标志物。
The gastrointestinal microbiota produces short-chain fatty acids, especially butyrate, which affect colonic health, immune function and epigenetic regulation. To assess the effects of nutrition and aging on the production of butyrate, the butyryl-CoA:acetate CoA-transferase gene and population shifts of Clostridium clusters lV and XlVa, the main butyrate producers, were analysed. Faecal samples of young healthy omnivores (24 +/- 2.5 years), vegetarians (26 +/- 5 years) and elderly (86 +/- 8 years) omnivores were evaluated. Diet and lifestyle were assessed in questionnaire-based interviews. The elderly had significantly fewer copies of the butyryl-CoA:acetate CoA-transferase gene than young omnivores (P=0.014), while vegetarians showed the highest number of copies (P=0.048). The thermal denaturation of the butyryl-CoA:acetate CoA-transferase gene variant melting curve related to Roseburia/Eubacterium rectale spp. was significantly more variable in the vegetarians than in the elderly. The Clostridium cluster XIVa was more abundant in vegetarians (P=0.049) and in omnivores (P < 0.01) than in the elderly group. Gastrointestinal microbiota of the elderly is characterized by decreased butyrate production capacity, reflecting increased risk of degenerative diseases. These results suggest that the butyryl-CoA:acetate CoA-transferase gene is a valuable marker for gastrointestinal microbiota function.