Application of an Oxygen Electrode to Evaluate Superoxide Anion-scavenging Ability
Application of an Oxygen Electrode to Evaluate Superoxide Anion-scavenging Ability
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DOI：
10.2116/analsci.26.903
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发表时间：
2010-08
影响因子：
1.6
通讯作者：
K. Komagoe;Hiroaki Takeuchi;Tsuyoshi Inoue;T. Katsu
中科院分区：
文献类型：
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作者：
K. Komagoe;Hiroaki Takeuchi;Tsuyoshi Inoue;T. Katsu
用氧电极测定了其清除超氧阴离子自由基(−)的能力。该方法基于氮三乙酸铁(III)与过氧化氢反应生成的−和清除反应降低−的浓度，通过电子转移反应将氧分子的生成转化为−(III)的变化。随着过氧化氢浓度的增加，氧的产生成比例地增加，而氧的产生则受到抑制，这取决于超氧化物歧化酶的浓度。因此，我们应用本反应体系来评价抗氧化剂清除−的能力，用氧电极测量氧产生的抑制程度。该方法与传统比色法具有良好的相关性，可通过监测硝基蓝四氮唑与−的反应来评价抗氧化剂清除−的活性。
The ability to scavenge superoxide anion radicals (^•O_2^−) was determined using an oxygen electrode. The method is based on the determination of ^•O_2^− generated by the reaction of nitrilotriacetatoiron(III) with hydrogen peroxide and a decrease in the concentration of ^•O_2^− by a scavenging reaction, converting into a change in the generation of oxygen molecules through an electron-transfer reaction from ^•O_2^− to nitrilotriacetatoiron(III). Oxygen generation, which enhanced proportionally with an increase in the concentration of hydrogen peroxide, was inhibited depending on the concentration of superoxide dismutase. Hence, we applied the present reaction system to evaluate the ^•O_2^−-scavenging abilities of an antioxidant, measuring the degree of inhibition of oxygen generation using an oxygen electrode. A good correlation was obtained between the present method and conventional colorimetry, monitoring the formation of blueformazan by the reaction of nitro blue tetrazolium with ^•O_2^−, to estimate the ^•O_2^−-scavenging activities of antioxidants.