Performance evaluation of detecting adenovirus by using rapid diagnostic kits among Japanese people
Performance evaluation of detecting adenovirus by using rapid diagnostic kits among Japanese people
复制标题
日本人使用快速诊断试剂盒检测腺病毒的性能评估
DOI：
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发表时间：
2012
影响因子：
2.2
通讯作者：
E. Goto
中科院分区：
文献类型：
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作者：
E. Goto
本研究评估了快速诊断试剂盒在日本人中检测腺病毒的诊断性能。进行了一项荟萃分析，以汇总免疫层析方法和乳胶凝集试验的灵敏度、特异性、阳性和阴性似然比以及诊断比值比;酶联免疫吸附试验已在另一项荟萃分析中进行了评价，尚未对其他诊断试剂盒进行适当研究。根据曲线下面积（0.961-0.991），免疫色谱法显示具有足够高的诊断能力，无论是否采集结膜或咽喉拭子或粪便标本;这显著高于采集结膜拭子时的酶联免疫吸附试验（P = 0.00）。此外，相对诊断优势比显示，通过粪便标本采样，免疫层析法的诊断能力高于乳胶凝集试验（P = 0.01）。此外，免疫层析法在采集咽拭子和粪便标本时具有接近确认水平的诊断能力（阳性似然比分别为28.0和43.6，阴性似然比分别为0.111和0.121），而酶联免疫吸附试验在采集结膜或咽拭子或粪便标本时不具有此特征。然而，即使对于免疫色谱法，结膜拭子采样时的合并灵敏度水平（0.667）也不可接受。因此，显而易见的是，在现有类型的快速检测方法中，免疫层析方法对于诊断腺病毒感染总体上是优选的，即使它们仍然不能通过阴性结果完全排除感染，特别是当对结膜拭子取样时。
This study evaluated the diagnostic performance of detecting adenovirus by rapid diagnostic kits among Japanese people. A meta-analysis was conducted to pool the sensitivity, specificity, positive and negative likelihood ratios, and diagnostic odds ratio of immunochromatography methods and latex agglutination tests; enzyme-linked immunosorbent assay tests had already been evaluated in another meta-analysis, and no other diagnostic kits have been appropriately studied. Immunochromatography methods were shown to have sufficiently high diagnostic power, regardless of whether conjunctiva or throat swabs, or stool specimens were sampled, based upon the area under the curve (0.961–0.991); this was significantly higher than enzyme-linked immunosorbent assay tests when sampling conjunctiva swabs (P = 0.00). Additionally, the relative diagnostic odds ratio showed a higher diagnostic power of immunochromatography methods than latex agglutination tests (P = 0.01) by sampling stool specimens. Furthermore, immunochromatography methods had a nearly confirmative level of diagnostic power when sampling throat swabs and stool specimens (positive likelihood ratio = 28.0 and 43.6, respectively, and negative likelihood ratio = 0.111 and 0.121, respectively), with enzyme-linked immunosorbent assay tests not sharing this characteristic when sampling either conjunctiva or throat swabs, or stool specimens. However, even regarding immunochromatography methods, the level of pooled sensitivity (0.667) when sampling conjunctiva swabs was not acceptable. It is apparent that, therefore, among the existing types of rapid detection methods, immunochromatography methods are overall preferable for diagnosing adenoviral infections, even though they still cannot completely rule out infections by negative results, especially when sampling conjunctiva swabs.