The Hepatitis E Virus Capsid C-Terminal Region Is Essential for the Viral Life Cycle: Implication for Viral Genome Encapsidation and Particle Stabilization
The Hepatitis E Virus Capsid C-Terminal Region Is Essential for the Viral Life Cycle: Implication for Viral Genome Encapsidation and Particle Stabilization
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DOI：
10.1128/jvi.00444-13
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发表时间：
2013-05-01
影响因子：
5.4
通讯作者：
Ishii, Koji
中科院分区：
文献类型：
--
作者：
Shiota, Tomoyuki;Li, Tian-Cheng;Ishii, Koji
戊型肝炎病毒（HEV）衣壳的c端52个氨基酸（C52aa）对形态并不是必需的，但C52aa编码区是复制所必需的。转染C52aa敲低突变体显示瞬时生长，最早的群体包括大多数非感染性（可能是空的）颗粒和少数具有c端衣壳降解的感染性颗粒。最后，可重复地生成完整的逆转。C52aa对病毒的生命周期至关重要，它能促进病毒囊化的精确，并稳定被囊化的颗粒。
Although the C-terminal 52 amino acids (C52aa) of hepatitis E virus (HEV) capsid are not essential for morphology, the C52aa-encoding region is required for replication. Transfection of a C52aa knockdown mutant showed transient growth, and the earliest population included a majority of noninfectious (possibly empty) particles and a minority of infectious particles with C-terminal capsid degradation. Finally, the complete revertant was generated reproducibly. C52aa is essential for the viral life cycle, promoting accurate encapsidation and stabilizing encapsidated particles.