Intestinal microbes derived butyrate is related to the immunomodulatory activities of Dendrobium officinale polysaccharide
Intestinal microbes derived butyrate is related to the immunomodulatory activities of Dendrobium officinale polysaccharide
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肠道微生物来源的丁酸盐与铁皮石斛多糖的免疫调节活性有关
DOI：
10.1016/j.ijbiomac.2020.01.305
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发表时间：
2020-04-15
影响因子：
8.2
通讯作者：
Ding, Kan
中科院分区：
文献类型：
--
作者：
Li, Meixia;Yue, Han;Ding, Kan
铁皮石斛多糖的免疫调节活性研究已有多年，但其肠道微生物代谢产物对免疫调节作用的贡献尚未见报道。从铁皮石斛（Dendrobium officinale Kimura et Migo）茎中分离得到平均分子量为39.4 kDa的多糖DOW-5 B。碳水化合物含量为91.97%，未检测到蛋白质。单糖分析表明，该多糖由葡萄糖醛酸和葡萄糖组成，摩尔比（M/G）为1.2：19.4。动物试验表明，DOW-5 B增加了小鼠肠道菌群的多样性。有益微生物如瘤胃球菌、真杆菌、梭菌、双歧杆菌、副拟杆菌和嗜粘蛋白阿克曼氏菌增加，而有害细菌变形菌减少。令人惊讶的是，DOW-5 B促进肠道微生物产生更多的丁酸盐，并且主要由Parabacteroides_sp_HGS0025产生。此外，我们发现大肠健康以及小鼠的免疫反应得到改善。此外，Parabacteroides_sp_HG50025分别与肠道和小鼠血液中的丁酸盐、IgM、IL-10和INF-α产物呈正相关。提示铁皮石斛多糖具有免疫调节作用，其作用可能是通过丁酸介导的。它增加了新的证据来支持草药多糖如何影响免疫力的基础。(C)2020 Elsevier B. V.保留所有权利。
Although immunomodulatory activities of Dendrobium officinale polysaccharide has been investigated for many years, yet the potential contribution of its metabolite derived from intestinal microbes on immunoregulation effect has not been reported. In this study, polysaccharide DOW-5B with average molecular weight of 39.4 kDa was isolated from the stem of Dendrobium officinale Kimura et Migo. The carbohydrate content was 91.97% and no protein was detected. The monosaccharide analysis showed this polysaccharide was composed of glucuronic acid and glucose at a molar ratio (M/G) of 1.2:19.4. Animal test indicated DOW-5B increased the diversity of gut microbiota on mice. Beneficial microbes such as Ruminococcus, Eubacterium, Clostridium, Bifidobacterium, Parabacteroides and Akkermansia muciniphila increased while harmful bacteria in Proteobacteria decreased. Surprisingly, DOW-5B promoted gut microbes to generate more butyrate and mainly produced by Parabacteroides_sp_HGS0025. Further, we found the health of large intestine as well as immunity response of mice was improved. In addition, Parabacteroides_sp_HG50025 positively correlated with butyrate, IgM, IL-10, and INF-alpha products in intestine and mice blood, respectively. The data suggested that Dendrobium officinale polysaccharide has function on immunity may be mediated by butyrate. It adds new evidence to support the basis of how herbal polysaccharides affect immunity. (C) 2020 Elsevier B.V. All rights reserved.