Decreased anchoring-fibril antigens (AF1 and AF2) in basal-cell carcinoma.
Decreased anchoring-fibril antigens (AF1 and AF2) in basal-cell carcinoma.
复制标题
基底细胞癌中锚定原纤维抗原（AF1 和 AF2）减少。
DOI：
10.1007/bf00510070
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发表时间：
1985
影响因子：
3
通讯作者：
Muhlbauer,JE
中科院分区：
文献类型：
--
作者：
Lane,AT;Goldsmith,LA;McCoon,PE;Muhlbauer,JE
基底细胞癌（BCC）中基底膜带（BMZ）成分发生改变。电子显微镜已在人BCC中鉴定出正常、不连续或不存在BMZ组分的区域[13，15，26]。在本研究中，我们使用了新的单克隆抗体的锚定纤维，研究这一组成部分的BMZ。对11例基底细胞癌患者的皮肤肿瘤（10例结节性，1例浅表性）和1例基底细胞痣综合征患者的掌窝[10]进行了大疱性类天疱疮抗原（BPA）、层粘连蛋白和单克隆抗体AF 1和AF 2识别的锚定原纤维抗原的检查[4，5]。将一个楔形肿瘤（1-2 mm）冷冻在最佳切割温度（OCT）化合物（Lab-Tek Products，纳珀维尔，IL，美国）中，并储存在-70 ℃下直至测定。使用Tissue-Tek-II冷冻切片机切割4微米厚的切片，并置于明胶制备的载玻片上。在染色之前，将组织切片风干20分钟。
Basement-membrane-zone (BMZ) components are altered in basal-cell carcinoma (BCC). Electron microscopy has identified areas of normal, discontinuous, or absent BMZ components in human BCC [13, 15, 26]. In the present study, we used new monoclonal antibodies to the anchoring fibrils to study this component of the BMZ. Skin tumors of 11 patients with BCC (10 nodular, 1 superficial) and the palmar pit of a patient with basal-cell nevus syndrome were examined [10] for bullous-pemphigoid antigen (BPA), laminin, and anchoring-fibril antigens recognized by monoclonal antibodies AF1 and AF2 [4, 5]. A wedge of tumor (1-2 mm) was frozen in optimal cutting temperature (OCT) compound (Lab-Tek Products, Naperville, IL, USA) and stored at-70~ until assayed. Fourmicron-thick sections were cut using a Tissue-Tek-II cryomicrotome and placed on gelatin-prepared slides. The tissue sections were air-dried for 20 rain before staining.