Influence of coronary architecture on the variability in myocardial infarction induced by coronary ligation in rats.
Influence of coronary architecture on the variability in myocardial infarction induced by coronary ligation in rats.
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冠状动脉结构对大鼠冠状动脉结扎引起的心肌梗塞变异性的影响。
DOI：
10.1371/journal.pone.0183323
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Sawa Y
中科院分区：
文献类型：
--
作者：
Kainuma S;Miyagawa S;Fukushima S;Tsuchimochi H;Sonobe T;Fujii Y;Pearson JT;Saito A;Harada A;Toda K;Shirai M;Sawa Y
研究表明，冠状动脉结扎技术造成的大鼠心肌梗塞的大小并不均匀，从4%到65%不等。我们假设冠状动脉结扎引起的梗死面积变异可能是由冠状动脉分支模式引起的。对50只正常Lewis大鼠和在左心耳正下方或左心耳远端2mm处结扎冠状动脉的慢性心肌梗死模型进行冠状动脉造影（各n = 25），然后进行组织学分析。与人类不同的是，大鼠有一条来自左冠状动脉（n = 30）或右冠状动脉（n = 20）近端部分的单一主要间隔动脉。左旋支动脉（LCX）有三种分支模式：33例（66%）的LCX从长的左主冠状动脉（LMCA）向外周分支，而其余17例（34%）的LCX从间隔动脉的近端部分或短的LMCA分支。无论 LCX 分支模式如何，远端冠状动脉结扎的大鼠均出现局限于前部区域的心肌梗塞。在近端冠状动脉结扎的大鼠中，64%（n = 16）有广泛的心肌梗塞，涉及前部和外侧区域，而其余的（36%，n = 9）则有局限于前部区域的心肌梗塞，完整的 LCX 起源于短 LMCA。由于其解剖位置，所有大鼠的室间隔均未发生梗塞。梗塞大小的变化不仅由结扎位点引起，而且还由不同的 LCX 分支模式引起。造成不同规模的心肌梗塞存在潜在风险，特别是针对大范围的心肌梗塞时。间隔动脉区域似乎总是免受冠状动脉结扎技术诱发的心肌梗塞的影响。
It has been shown that the size of myocardial infarction in rats created by coronary ligation technique is not uniform, varying from 4% to 65%. We hypothesized that infarct size variability induced by coronary artery ligation might be caused by coronary artery branching pattern. Coronary artery angiography was performed in 50 normal Lewis rats and in chronic myocardial infarction models in which coronary artery was ligated immediately below the left atrial appendage or 2mm distal to the left atrial appendage (n = 25 for each), followed by histological analysis. Unlike the human, the rats had a single major septal artery arising from the proximal part of the left coronary artery (n = 30) or right coronary artery (n = 20). There were three branching patterns of left circumflex artery (LCX): 33 (66%) had LCX branching peripherally from a long left main coronary artery (LMCA), while the remainder 17 (34%) had the LCX branching from the proximal part of the septal artery or a short LMCA. The rats with distal coronary ligation presented myocardial infarction localized to an anterior territory irrespective of LCX branching pattern. In the rats with proximal coronary ligation, 64% (n = 16) had broad myocardial infarction involving the anterior and lateral territories, while the remainder (36%, n = 9) had myocardial infarction localized to an anterior territory with the intact LCX arising proximally from a short LMCA. The interventricular septum was spared from infarction in all rats because of its anatomical location. Infarct size variations were caused not only by ligation site but also by varying LCX branching patterns. There are potential risks to create different sizes of myocardial infarction, particularly when targeting a broad range of myocardial infarction. The territory of the septal artery always appears to be spared from myocardial infarction induced by the coronary ligation technique.