Highly parallel SNP genotyping
Highly parallel SNP genotyping
复制标题
DOI：
10.1101/sqb.2003.68.69
复制
发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Chee, MS
中科院分区：
文献类型：
--
作者：
Fan, JB;Oliphant, A;Chee, MS
用于常规微阵列的扫描仪通常具有3-5微米范围内的成像光点尺寸，不足以分辨随机组装的光纤阵列上的~5微米间距的特征。因此，我们开发了一种紧凑的共焦型成像系统，分辨率为~0.8μm，双激光照射(532 nm和635 nm)。2003年)。这种扫描仪能够在大约1.5小时内在两个颜色通道中对96个阵列矩阵成像，这使得每个扫描仪每天可以处理大约8-10个阵列矩阵，对应于大约100万个基因类型。
Scanners for conventional microarrays typically have imaging spot sizes in the range of 3–5 microns, insufficient to resolve the~ 5-micron-spaced features on the randomly assembled optical fiber-based arrays. We therefore developed a compact confocal-type imaging system with~ 0.8-μm resolution and two-laser illumination (532 and 635 nm; Barker et al. 2003). This scanner is able to image a 96-array matrix in both color channels in about 1.5 hours, which allows a throughput of~ 8–10 array matrices, corresponding to~ 1 million genotypes, per scanner per day.