Sex-specific activity and function of hypothalamic nonapeptide neurons during nest-building in zebra finches
Sex-specific activity and function of hypothalamic nonapeptide neurons during nest-building in zebra finches
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DOI：
10.1016/j.yhbeh.2013.10.001
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发表时间：
2013-11-01
影响因子：
3.5
通讯作者：
Goodson, James L.
中科院分区：
文献类型：
--
作者：
Klatt, James D.;Goodson, James L.
从鱼类到人类的脊椎动物物种参与了一系列复杂的准备行为，称为筑巢;然而，尽管其系统发育普遍存在，但筑巢背后的生理和神经机制并不为人所知。我们在这里测试的假设，筑巢行为的影响，加压素-催产素（VP-OT）肽，根据他们在哺乳动物的父母的行为中发挥的作用。我们定量筑巢行为的雄性和雌性斑胸草雀外周和中央管理的OT和Via受体（OTR和VlaR，分别）拮抗剂。外周注射的OTR拮抗剂大大减少筑巢行为的女性，但不是男性，而类似的注射VlaR拮抗剂产生相对温和的影响，在两种性别。然而，中枢拮抗剂输注对筑巢不产生影响，并且OTR拮抗剂注射到乳房中产生的作用显著弱于注射到腹股沟区中的作用，这表明拮抗剂作用可能通过输卵管在外周介导。最后，免疫细胞化学被用来量化嵌套诱导的Fos激活的九肽神经元的室旁核和视上核的下丘脑和内侧床核的终纹。筑巢诱导Fos的表达在室旁VP神经元的女性，但不是男性。因为OT（Ile（8）-OT;中催产素）和VP（Ile（3）-VP;催产素）的禽类形式对禽类OTR表现出高亲和力，并且因为这两种肽形式调节子宫收缩力，我们假设筑巢相关刺激诱导室旁催产素神经元释放肽，然后通过输卵管子宫中OTR结合的外周反馈促进雌性筑巢。(C)版权所有© 2013 Elsevier Inc.
Vertebrate species from fish to humans engage in a complex set of preparatory behaviors referred to as nesting; yet despite its phylogenetic ubiquity, the physiological and neural mechanisms that underlie nesting are not well known. We here test the hypothesis that nesting behavior is influenced by the vasopressin-oxytocin (VP-OT) peptides, based upon the roles they play in parental behavior in mammals. We quantified nesting behavior in male and female zebra finches following both peripheral and central administrations of OT and Via receptor (OTR and VlaR, respectively) antagonists. Peripheral injections of the OTR antagonist profoundly reduce nesting behavior in females, but not males, whereas comparable injections of VlaR antagonist produce relatively modest effects in both sexes. However, central antagonist infusions produce no effects on nesting, and OTR antagonist injections into the breast produce significantly weaker effects than those into the inguinal area, suggesting that antagonist effects are mediated peripherally, likely via the oviduct. Finally, immunocytochemistry was used to quantify nesting-induced Fos activation of nonapeptide neurons in the paraventricular and supraoptic nuclei of the hypothalamus and the medial bed nucleus of the stria terminalis. Nest-building induced Fos expression within paraventricular VP neurons of females but not males. Because the avian forms of OT (Ile(8)-OT; mesotocin) and VP (Ile(3)-VP; vasotocin) exhibit high affinity for the avian OTR, and because both peptide forms modulate uterine contractility, we hypothesize that nesting-related stimuli induce peptide release from paraventricular vasotocin neurons, which then promote female nesting via peripheral feedback from OTR binding in the oviduct uterus. (C) 2013 Elsevier Inc All rights reserved.