Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury
Upregulation of matrix metalloproteinase 9 (MMP9)/tissue inhibitor of metalloproteinase 1 (TIMP1) and MMP2/TIMP2 ratios may be involved in lipopolysaccharide-induced acute lung injury
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DOI：
10.1177/0300060520919592
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发表时间：
2020-04-01
影响因子：
1.6
通讯作者：
Ma, Qilin
中科院分区：
文献类型：
--
作者：
Chen, Guobing;Ge, Dandan;Ma, Qilin
目的探讨内毒素（LPS）诱导的急性肺损伤（ALI）大鼠肺组织中基质金属蛋白酶9（MMP 9）、基质金属蛋白酶2（MMP 2）、基质金属蛋白酶组织抑制因子1（TIMP 1）和基质金属蛋白酶组织抑制因子2（TIMP 2）的变化及意义（注射生理盐水）和ALI组（注射LPS），然后再分为4个时间点（2、6、12和24小时）。ELISA法检测血清肿瘤坏死因子α和白细胞介素6水平，观察LPS注射后的炎症反应。通过肺组织苏木精-伊红染色、肺湿/干重比、肺通透性指数等指标判断ALI程度。结果MMP 9/TIMP 1和MMP 2/TIMP 2的变化与肺湿/干重比、肺通透性指数、肺组织病理学改变、肺组织病理学改变、肺组织结论LPS诱导的ALI可能与MMP 9/TIMP 1、MMP 2/TIMP 2比值升高有关。
ObjectiveThis study aimed to examine the changes and significance of matrix metalloproteinase 9 (MMP9), MMP2, tissue inhibitor of metalloproteinase 1 (TIMP1), and TIMP2 in rats with lipopolysaccharide (LPS)-induced acute lung injury (ALI).MethodsWistar rats were randomly divided into a control group (injected with saline) and an ALI group (injected with LPS), then subdivided into four time points (2, 6, 12, and 24 hours). Serum tumor necrosis factor alpha and interleukin-6 levels were detected by ELISA to investigate the inflammatory reaction after LPS injection. The degree of ALI was determined by hematoxylin-eosin staining of lung tissue, the lung wet/dry weight ratio, and pulmonary permeability index. Changes in lung MMP and TIMP protein and mRNA levels were detected by western blotting and quantitative real-time polymerase chain reaction.ResultsChanges in the ratios of MMP9/TIMP1 and MMP2/TIMP2 were consistent with and strongly positively associated with the lung wet/dry weight ratio, the pulmonary permeability index, and serum tumor necrosis factor alpha and interleukin-6 levels in the ALI group.ConclusionALI induced by LPS may be related to upregulation of MMP9/TIMP1 and MMP2/TIMP2 ratios.