The effects of cold, light and time of day during low-temperature shift on the expression of CBF6, FpCor14b and LOS2 in Festuca pratensis
The effects of cold, light and time of day during low-temperature shift on the expression of CBF6, FpCor14b and LOS2 in Festuca pratensis
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DOI：
10.1016/j.plantsci.2011.08.004
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发表时间：
2012-02-01
期刊：
影响因子：
5.2
通讯作者：
Sasal, Monika
中科院分区：
文献类型：
--
作者：
Jurczyk, Barbara;Rapacz, Marcin;Sasal, Monika
严格控制和协调诱导 CBF 调节子（一组受 CBF 蛋白调节的基因）可促进植物的耐冻性。 CBF 调节 COR 基因的表达，从而赋予耐冻性。大麦中的 COR14b 是效应基因之一，在抵抗光合作用的联合冷冻和光抑制方面似乎很重要。 LOS2 抑制 STS/ZAT10（CBF 靶基因的负调节因子）的转录，从而充当 COR 基因的正调节因子。据报道，在拟南芥中，CBF 的低温诱导是由生物钟控制的。此外，光质量信号已被证明可以调节一些植物的耐冻基因。我们研究的目的是确定光和冷联合处理对羊茅 CBF6、FpCor14b 和 LOS2 基因转录水平的影响。我们已经证明了 CBF6 的调节。草地早熟禾中的 FpCor14b 和 LOS2 诱导动力学是通过低温转变过程中温度和光与一天中时间的相互作用而发生的。 FpCOR14b 转录水平显示出通过增加光强度而上调。还证明光质量对CBF6具有强烈的调节作用。 FpCor14b 和 LOS2 转录低温下的诱导动力学。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
Strictly controlled and coordinated induction of CBF regulon (a set of genes regulated by CBF proteins) promotes plant freezing tolerance. CBFs regulate the expression of COR genes that confer freezing tolerance. COR14b in barley is one of the effector genes which seems to be important in resistance to combined freezing and photoinhibition of photosynthesis. LOS2 represses the transcription of STS/ZAT10 (a negative regulator of CBF-target genes) and thus acts as a positive regulator of COR genes. In Arabidopis, low temperature induction of CBFs was reported to be gated by the circadian clock. Moreover, light-quality signals have been shown to regulate some plants' freezing tolerance genes. The aim of our study was to determine the effects of combined treatment with light and cold on the transcript levels of CBF6, FpCor14b and LOS2 genes in Festuca pratensis. We have demonstrated that the regulation of CBF6. FpCor14b and LOS2 induction kinetics in F. pratensis occurs through the interaction of temperature and light with time of day during low-temperature shift. The FpCOR14b transcript level was shown to be up-regulated by increasing light intensity. It was also proved that light quality strongly regulates CBF6. FpCor14b and LOS2 transcripts induction kinetics at low temperatures. (C) 2011 Elsevier Ireland Ltd. All rights reserved.