Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS
Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS
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DOI：
10.1194/jlr.d007567
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发表时间：
2010-09-01
影响因子：
6.5
通讯作者：
Liu, Huwei
中科院分区：
文献类型：
--
作者：
Nie, Honggang;Liu, Ranran;Liu, Huwei
开发了一种用于大鼠腹膜表面层脂质分析的在线二维（2D）液相色谱（LC）四极杆飞行时间质谱（QToF-MS）方法，其中脂质类和种类可以在一次注射中同时分离，灵敏度显著提高。在第一维中，正相柱分离不同的脂类，在第二维中，反相柱分离脂类分子，从而在提高检测灵敏度的同时降低了离子抑制效应。共鉴定出12类721种内源性脂质分子，其中415种通过串联质谱进行了结构鉴定，另外306种通过精确质量鉴定。线性、检出限和重复性均符合要求。将该方法应用于大鼠腹膜透析后腹膜表面层脂质变化的研究，共鉴定出32种潜在的脂质生物标志物，给药组的浓度是对照组的2.2-12.5倍。结果表明，这种2D LC-MS系统是复杂生物样品脂质分析的一种有前途的工具。Nie，H.，R. Liu，Y. Yang，Y. Bai，Y. Guan、黑腹叶蝉D.钱氏T. Wang和H.刘某通过在线正相和反相2D LC QToF-MS对大鼠腹膜表面层进行脂质分析。J. Lipid Res. 2010。51：2833-2844。
An online, two-dimensional (2D) liquid chromatography (LC) quadrupole time-of-flight mass spectrometry (QToF-MS) method was developed for lipid profiling of rat peritoneal surface layers, in which the lipid classes and species could be simultaneously separated in one injection with a significantly increased sensitivity. Different lipid classes were separated on a normal-phase column in the first dimension and lipid molecular species were separated on a reversed-phase column in the second dimension, so that the ion suppression effects were reduced while the detection sensitivity was improved. Identified were 721 endogenous lipid species from 12 lipid classes, in which 415 structures were confirmed using tandem mass spectra, and the other 306 lipid molecular species were identified by accurate masses. The linearity, limit of detection, and repeatability were all satisfactory. The method was applied to the investigation of the lipid changes in rat peritoneal surface layer after peritoneal dialysis, and 32 potential lipid biomarkers were identified, as their concentrations in the dosed group were 2.2-12.5 times of those in the control group. The results revealed that this 2D LC-MS system was a promising tool for lipid profiling of complex biological samples.-Nie, H., R. Liu, Y. Yang, Y. Bai, Y. Guan, D. Qian, T. Wang, and H. Liu. Lipid profiling of rat peritoneal surface layers by online normal-and reversed-phase 2D LC QToF-MS. J. Lipid Res. 2010. 51: 2833-2844.