Infectious bronchitis virus S2 gene sequence variability may affect S1 subunit specific antibody binding
Infectious bronchitis virus S2 gene sequence variability may affect S1 subunit specific antibody binding
复制标题
DOI：
10.1023/a:1008179208217
复制
发表时间：
1999-10-01
期刊：
影响因子：
1.6
通讯作者：
Hilt, DA
中科院分区：
文献类型：
--
作者：
Callison, SA;Jackwood, MW;Hilt, DA
测定了几株属于阿肯色州、康涅狄格州和佛罗里达血清型的传染性支气管炎病毒（IBV）的S2基因。系统发育分析的S2基因核苷酸和推导的氨基酸序列数据导致组的菌株是相同的分组时观察到的S1序列数据。因此，似乎S2亚基在血清型内是保守的，但在血清型之间不是。虽然序列差异很小，我们发现，只有少数氨基酸的差异是负责不同的二级结构预测的S2亚基。这些变化可能在S1和S2亚基之间产生不同的相互作用，这可能影响血清型特异性表位所在的S1亚基的构象。基于此序列数据，我们假设S2亚基可以影响特异性抗体与IBV刺突糖蛋白S1亚基的结合。
The S2 gene of several strains of infectious bronchitis virus (IBV) belonging to the Arkansas, Connecticut, and Florida serotypes was sequenced. Phylogenetic analysis of the S2 gene nucleotide and deduced amino acid sequence data resulted in groups of strains that were the same as groupings observed when S1 sequence data was used. Thus, it appears that S2 subunits are conserved within a serotype but not between serotypes. Although the sequence differences were small, we found that only a few amino acid differences were responsible for different secondary structure predictions for the S2 subunit. It is likely that these changes create different interactions between the S1 and S2 subunits, which could affect the conformation of the S1 subunit where serotype specific epitopes are located. Based on this sequence data, we hypothesize that the S2 subunit can affect specific antibody binding to the S1 subunit of the IBV spike glycoprotein.