Chronic Stress Shifts Effort-Related Choice Behavior in a Y-Maze Barrier Task in Mice.
Chronic Stress Shifts Effort-Related Choice Behavior in a Y-Maze Barrier Task in Mice.
复制标题
DOI：
10.3791/61548
复制
发表时间：
2020-08-13
期刊：
影响因子：
--
通讯作者：
Samuels BA
中科院分区：
文献类型：
--
作者：
Dieterich A;Yohn CN;Samuels BA
情绪障碍，包括重度抑郁症，可由慢性压力诱发。y型迷宫障碍任务是一个与努力相关的选择测试，它衡量的是付出努力和获得奖励的动机。在小鼠中，与没有压力的小鼠相比，慢性压力暴露显著影响了小鼠在自由获得较小价值奖励时为更高价值奖励而工作的动机。在这里，我们描述了慢性皮质酮给药范式，它在y型迷宫屏障任务中产生努力反应的转变。在y型迷宫任务中，一只手臂中有4个食物颗粒，而另一只手臂中只有2个颗粒。在小鼠学会选择高奖励组后，在多个测试阶段将高度逐渐增加的障碍物引入高奖励组。不幸的是，大多数慢性应激范式（包括皮质酮和社会失败）是在雄性小鼠中发展起来的，在雌性小鼠中效果较差。因此，我们还讨论了慢性非歧视性社会失败压力（CNSDS），这是我们开发的一种压力范式，在雄性和雌性小鼠中都有效。在雄性和雌性小鼠中使用多种不同的慢性应激源重复结果，并增加翻译相关行为任务的使用，将有助于促进对慢性应激如何导致情绪障碍的理解。y形迷宫障碍任务是一种行为测试，检验人们为获得奖励而付出努力的动机。在这里，我们讨论了用这种行为测试多种有效的慢性应激源，包括慢性皮质酮和社会失败应激，以及在女性中有效的新型慢性非歧视性社会失败应激（CNSDS）。
Mood disorders, including major depressive disorder, can be precipitated by chronic stress. The Y-maze barrier task is an effort-related choice test that measures motivation to expend effort and obtain reward. In mice, chronic stress exposure significantly impacts motivation to work for a higher value reward when a lesser value reward is freely available compared to unstressed mice. Here we describe the chronic corticosterone administration paradigm, which produces a shift in effortful responding in the Y-maze barrier task. In the Y-maze task, one arm contains 4 food pellets, while the other arm contains only 2 pellets. After mice learn to select the high reward arm, barriers with progressively increasing height are then introduced into the high reward arm over multiple test sessions. Unfortunately, most chronic stress paradigms (including corticosterone and social defeat) were developed in male mice and are less effective in female mice. Therefore, we also discuss chronic non-discriminatory social defeat stress (CNSDS), a stress paradigm we developed that is effective in both male and female mice. Repeating results with multiple distinct chronic stressors in male and female mice combined with increased usage of translationally relevant behavior tasks will help to advance the understanding of how chronic stress can precipitate mood disorders. The Y-maze barrier task is a behavior test that examines motivation to expend effort for reward. Here, we discuss testing multiple well-validated chronic stressors including chronic corticosterone and social defeat stress with this behavior, as well as the novel chronic non-discriminatory social defeat stress (CNSDS), which is effective in females.