Protective effects of seabuckthorn pulp and seed oils against radiation-induced acute intestinal injury.
Protective effects of seabuckthorn pulp and seed oils against radiation-induced acute intestinal injury.
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DOI：
10.1093/jrr/rrw069
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发表时间：
2017-01
影响因子：
2
通讯作者：
Sun W
中科院分区：
文献类型：
--
作者：
Shi J;Wang L;Lu Y;Ji Y;Wang Y;Dong K;Kong X;Sun W
辐射引起的胃肠道综合征，包括恶心、腹泻和脱水，是医疗或工业辐射照射后发病率和死亡率的原因之一。目前尚无安全有效的放射防护措施被批准用于临床治疗。在这项研究中，我们的目的是探讨潜在的保护作用，沙棘果肉和种子油对辐射引起的急性肠道损伤。C57/BL 6小鼠在暴露于7.5戈伊的全身X射线照射之前，每天一次经口给予沙棘果肉油、种子油和对照橄榄油，持续7天。采用末端脱氧核苷酸转移酶dUTP缺口末端标记、实时定量聚合酶链反应和蛋白质印迹法检测凋亡细胞和蛋白、炎症因子和丝裂原活化蛋白（MAP）激酶。沙棘油预处理可提高辐射后小鼠的存活率，减少小肠绒毛的损伤面积。果肉和种子油处理均显著降低凋亡细胞数和切割的caspase 3表达。沙棘油可下调肿瘤坏死因子-α、白细胞介素（IL）-1β、IL-6和IL-8等炎症因子的mRNA水平。果肉和种子油都提高了磷酸化细胞外信号调节激酶的水平，降低了磷酸化c-Jun N-末端激酶和p38的水平。棕榈油酸（PLA）和α亚麻酸（ALA）分别是果肉油和种子油的主要成分。PLA和ALA预处理可延长放疗后的生存时间。总之，沙棘果肉和种子油通过减少细胞凋亡和炎症来保护小鼠免受高剂量辐射引起的肠道损伤。ALA和PLA是有希望的天然辐射对抗候选者。
Radiation-induced gastrointestinal syndrome, including nausea, diarrhea and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure. No safe and effective radiation countermeasure has been approved for clinical therapy. In this study, we aimed to investigate the potential protective effects of seabuckthorn pulp and seed oils against radiation-induced acute intestinal injury. C57/BL6 mice were orally administered seabuckthorn pulp oil, seed oil and control olive oil once per day for 7 days before exposure to total-body X-ray irradiation of 7.5 Gy. Terminal deoxynucleotidyl transferase dUTP nick end labeling, quantitative real-time polymerase chain reaction and western blotting were used for the measurement of apoptotic cells and proteins, inflammation factors and mitogen-activated protein (MAP) kinases. Seabuckthorn oil pretreatment increased the post-radiation survival rate and reduced the damage area of the small intestine villi. Both the pulp and seed oil treatment significantly decreased the apoptotic cell numbers and cleaved caspase 3 expression. Seabuckthorn oil downregulated the mRNA level of inflammatory factors, including tumor necrosis factor-α, interleukin (IL)-1β, IL-6 and IL-8. Both the pulp and seed oils elevated the level of phosphorylated extracellular-signal-regulated kinase and reduced the levels of phosphorylated c-Jun N-terminal kinase and p38. Palmitoleic acid (PLA) and alpha linolenic acid (ALA) are the predominant components of pulp oil and seed oil, respectively. Pretreatment with PLA and ALA increased the post-radiation survival time. In conclusion, seabuckthorn pulp and seed oils protect against mouse intestinal injury from high-dose radiation by reducing cell apoptosis and inflammation. ALA and PLA are promising natural radiation countermeasure candidates.