Integrated hand-powered centrifugation and paper-based diagnosis with blood-in/answer-out capabilities
Integrated hand-powered centrifugation and paper-based diagnosis with blood-in/answer-out capabilities
复制标题
集成手动离心和纸质诊断，具有血液输入/应答输出功能
DOI：
10.1016/j.bios.2020.112282
复制
发表时间：
2020-10-01
影响因子：
12.6
通讯作者：
Chen, Lingxin
中科院分区：
文献类型：
--
作者：
Li, Bowei;Qi, Ji;Chen, Lingxin
迄今为止，发展中国家55%的居民仍然生活在农村地区，他们非常迫切需要提高疾病诊断水平，特别是在资源有限的地区。在此，我们提出了一种微流体系统，可以快速、可行和集成的方式离心全血并量化所含的生物标志物。这种设备提供血液输入/应答输出功能，并且足够小，可以由任何人在任何环境中携带。手动离心机和免疫分析装置成功集成在旋转纸基设备中，允许未经培训的用户在无法获得电力的环境中进行诊断应用。此外，成本低（约 0.5 美元）、重量轻且仪器占用空间小，使得该设备非常适合快速现场检测。为了验证该系统在临床诊断测试中的适用性，我们成功地对人体血液样本中的癌胚抗原和甲胎蛋白进行了酶联免疫吸附测定（ELISA）分析。我们期望这种强大的平台技术将为资源有限的环境中的即时诊断提供机会。
To date, 55% of inhabitants of the developing world still live in rural regions and they have a very urgent need to improve the level of disease diagnosis, especially in resource-limited settings. Herein, we present a microfluidic system that centrifuges whole blood and quantifies contained biomarkers in a rapid, feasible and integrated way. Such a device provides a blood-in/answer-out capability and is small enough to be carried by any individual in any environment. The successful integration a hand-powered centrifuge and immunoassay unit within a rotational paper-based device allows for diagnostic application by untrained users and in environments where access to electricity cannot be assumed. In addition, the low cost (similar to$ 0.5), light weight and small instrumental footprint make the device ideally suited for rapid on-site detection. To validate the applicability of the system in a clinical diagnostic testing, we successfully perform enzyme-linked immunosorbent assay (ELISA) analysis of carcinoembryonic antigen and alpha fetoprotein from human blood samples. We expect that this powerful platform technology will provide the opportunities for point-of-care diagnosis in resource-limited settings.