The anti-allergic effect of the ethyl acetate fraction from an Ecklonia kurome extract.
The anti-allergic effect of the ethyl acetate fraction from an Ecklonia kurome extract.
复制标题
黑昆布提取物中的乙酸乙酯部分的抗过敏作用。
DOI：
10.1080/09540105.2014.880665
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
R. Tanaka and T. Matsushita
中科院分区：
文献类型：
--
作者：
Y. Sugiura;K. Nagayama;Y. Kinoshita;R. Tanaka and T. Matsushita
目的：研究黑珊瑚乙酸乙酯部位的抗过敏作用。比较了黑色素（EEK）提取物与来自安子香提取物的乙酸乙酯级分（EEA）和表没食子儿茶素没食子酸酯（EGCG）对实验动物中的炎症、炎性淋巴细胞中的脱粒和涉及过敏反应的酶活性的抑制作用。发现EEK通过局部和口服给药以剂量依赖性方式抑制炎症诱导剂（花生四烯酸、12-O-十四酰基佛波醇-13-乙酸酯和恶唑酮）引起的小鼠耳水肿。EEK还以剂量依赖性方式抑制大鼠嗜碱性白血病细胞的脱粒和酶活性（环氧合酶-2、大豆脂氧合酶和磷脂酶A2）。EEK的效果与EEA和EGCG的效果大致相当。因此，E.黑米和EEK中的成分不仅可以用于普通食品，而且可以作为功能性食品资源更广泛地用于抗过敏食品。
To determine the anti-allergic effects ofEcklonia kurome, the ethyl acetate fraction from anE. kurome(EEK) extract was compared to the ethyl acetate fraction from anEisenia arborea(EEA) extract and epigallocatechin gallate (EGCG) for inhibitory effects on inflammation in experimental animals, degranulation in inflammatory lymphocytes and enzymatic activities involved in allergic reactions. EEK was found to inhibit mouse ear oedema by inflammatory inducers (arachidonic acid, 12-O-tetradecanoylphorbol-13-acetate and oxazolone) in a dose-dependent manner, through both topical and oral administrations. EEK also inhibited degranulation in rat basophilic leukaemia cells and enzymatic activities (cyclooxygenase-2, soybean lipoxygenase and phospholipase A2) in a dose-dependent manner. The effects of EEK were generally comparable of those of EEA and EGCG. Therefore,E. kuromeand the components in EEK have the benefit of being used not only in common foods, but also more widely as a functional food resource in anti-allergic foods.