LIGAND-RECEPTOR INTERACTIONS IN THE PHAGOCYTOSIS OF VIRULENT STREPTOCOCCUS-PNEUMONIAE BY POLYMORPHONUCLEAR LEUKOCYTES
LIGAND-RECEPTOR INTERACTIONS IN THE PHAGOCYTOSIS OF VIRULENT STREPTOCOCCUS-PNEUMONIAE BY POLYMORPHONUCLEAR LEUKOCYTES
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DOI：
10.1093/infdis/154.4.619
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发表时间：
1986-10-01
影响因子：
6.4
通讯作者：
HOSTETTER, MK
中科院分区：
文献类型：
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作者：
GORDON, DL;JOHNSON, GM;HOSTETTER, MK
我们使用中性粒细胞补体受体CR 1和CR 3的多克隆和单克隆抗体来评估这些受体在吞噬毒性肺炎链球菌噬菌体3、6A和14中的作用，所述噬菌体3、6A和14具有与多糖囊共价结合的可接近的C3配体。当用单克隆抗体OKM 10阻断正常多形核白细胞（PMNLs）上的iC 3b受体（CR 3）时，在混合的人血清中对6A和14型肺炎球菌（只携带iC 3b）的吞噬作用被抑制50%-80%，在非免疫血清中则完全被抑制。阻断PMNL C3 b受体（CR 1）未能抑制血清型6A和14的吞噬作用。对于在荚膜上携带C3 b和C3 d（以及iC 3b）的血清型3，CR 3介导的吞噬作用仅占摄取的20%;同样，没有证据表明CR 1介导的吞噬作用。iC 3b配体引起了一贯更大的释放超氧化物，髓过氧化物酶，和乳铁蛋白比C3 b。因此，iC 3b/CR 3相互作用是携带iC 3b的肺炎球菌的吞噬作用和刺激细胞内杀菌过程的主要触发因素。
We used polyclonal and monoclonal antibodies to neutrophil complement receptors CR1 and CR3 to assess the role of these receptors in the phagocytosis of virulent Streptococcus pneumoniae serotyes 3, 6A, and 14, which bear accessible C3 ligands covalently bound to the polysaccharide capsule. When the iC3b receptor (CR3) on normal polymorphonuclear leukocytes (PMNLs) was blocked by the monoclonal antibody OKM10, phagocytosis of pneumococcal serotypes 6A and 14 (which bear exclusively iC3b) was inhibited 50%-80% in pooled human serum and completely in nonimmune serum. Blockade of the PMNL C3b receptor (CR1) failed to inhibit phagocytosis for sertypes 6A and 14. For serotype 3, which bears C3b and C3d (as well as iC3b) on the capsule, CR3-mediated phagocytosis accounted for only 20% of the uptake; again, there was no evidence for CR1-mediated phagocytosis. The iC3b ligand elicited consistently greater release of superoxide, myeloperoxidase, and lactoferrin than did C3b. The iC3b/CR3 interaction is thus the primary trigger for phagocytosis of iC3b-bearing pneumococci and for stimulation of intracellular bactericidal processes.