DICER and ZRF1 contribute to chromatin decondensation during nucleotide excision repair.
DICER and ZRF1 contribute to chromatin decondensation during nucleotide excision repair.
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DOI：
10.1093/nar/gkx261
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发表时间：
2017-06-02
影响因子：
14.9
通讯作者：
Richly H
中科院分区：
文献类型：
--
作者：
Chitale S;Richly H
受损DNA的修复依赖于DNA修复因子以精心策划的方式的募集。作为先决条件，染色质需要通过染色质重塑剂去致密化，以允许修复因子的结合和DNA修复发生。最近的研究表明，SWI/SNF和INO 80家族的成员以及PARP 1参与了核苷酸切除修复（NER）。在这项研究中，我们报告说，内切核酸酶DICER涉及染色质去浓缩过程中NER。响应于UV照射，DICER以ZRF 1介导的方式被募集到染色质。H2 A-泛素结合蛋白ZRF 1和DICER通过PARP 1共同影响染色质构象。此外，DICER介导的染色质去凝聚与其催化活性无关。两者合计，我们描述了一个新的功能，在染色质和它的相互作用与泛素信号级联在GG-NER。
Repair of damaged DNA relies on the recruitment of DNA repair factors in a well orchestrated manner. As a prerequisite, the chromatin needs to be decondensed by chromatin remodelers to allow for binding of repair factors and for DNA repair to occur. Recent studies have implicated members of the SWI/SNF and INO80 families as well as PARP1 in nucleotide excision repair (NER). In this study, we report that the endonuclease DICER is implicated in chromatin decondensation during NER. In response to UV irradiation, DICER is recruited to chromatin in a ZRF1-mediated manner. The H2A–ubiquitin binding protein ZRF1 and DICER together impact on the chromatin conformation via PARP1. Moreover, DICER-mediated chromatin decondensation is independent of its catalytic activity. Taken together, we describe a novel function of DICER at chromatin and its interaction with the ubiquitin signalling cascade during GG-NER.