A newly identified protein complex that mediates white spot syndrome virus infection via chitin-binding protein
A newly identified protein complex that mediates white spot syndrome virus infection via chitin-binding protein
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DOI：
10.1099/vir.0.064782-0
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发表时间：
2014-08-01
影响因子：
3.8
通讯作者：
Chen, Li-Li
中科院分区：
文献类型：
--
作者：
Huang, Po-Yu;Leu, Jiann-Horng;Chen, Li-Li
白斑综合征病毒（WSSV）是一种大包膜病毒，给对虾养殖业造成了严重的死亡和巨大的经济损失。被包膜的病毒必须通过病毒包膜蛋白与宿主细胞膜的受体结合。在WSSV中，已有多项研究发现了包膜蛋白与宿主细胞膜受体的结合，如VP53A等10种蛋白与几丁质结合蛋白（CBP）结合，VP28与单对虾Rab7结合，VP187与β -整合素结合等。WSSV包膜蛋白也被认为能够形成一种被称为“感染组”的蛋白质复合物。本研究重点研究了CBP在WSSV感染过程中的作用，以及CBP与包膜蛋白VP24、VP28、VP31、VP32、VP39B、VP53A和VP56之间的关系。反转录pcr分析结果表明，CBP存在于多种对虾中。抑制CBP基因的表达可减缓WSSV的感染速度。进行了Far-Western blot分析和His pull-down实验，发现一个蛋白复合物似乎由一个“连接”蛋白组成，该蛋白由VP31、VP32和VP39B以及四个包膜蛋白组成，包括VP24、VP28、VP53A和VP56。该蛋白复合物可能是感染组的另一部分，也可能是与CBP结合的区域。这项研究的发现可能为进一步的WSSV研究确定了某些要点。
White spot syndrome virus (WSSV) is a large enveloped virus which has caused severe mortality and huge economic losses in the shrimp farming industry. The enveloped virus must be combined with the receptors of the host cell membrane by the virus envelope proteins. In the case of WSSV, binding of envelope proteins with receptors of the host cell membrane was discovered in a number of previous studies, such as VP53A and 10 other proteins with chitin-binding protein (CBP), VP28 with Penaeus monodon Rab7, VP187 with beta-integrin, and so on. WSSV envelope proteins were also considered capable of forming a protein complex dubbed an 'infectome'. In this study, the research was focused on the role of CBP in the WSSV infection process, and the relationship between CBP and the envelope proteins VP24, VP28, VP31, VP32 VP39B, VP53A and VP56. The results of the reverse transcription-PCR analyses showed that CBP existed in a variety of shrimp. The speed of WSSV infection could be slowed down by inhibiting CBP gene expression. Far-Western blot analysis and His pull-down assays were conducted, and a protein complex was found that appeared to be composed of a 'linker' protein consisting of VP31, VP32 and VP39B together with four envelope proteins, including VP24, VP28, VP53A and VP56. This protein complex was possibly another part of the infectome and the possible binding region with CBP. The findings of this study may have identified certain points for further WSSV research.