Characterization of accessions of 'Reine Claude Verte' plum using Prunus SRR and phenotypic traits
Characterization of accessions of 'Reine Claude Verte' plum using Prunus SRR and phenotypic traits
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DOI：
10.1016/j.scienta.2014.02.018
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发表时间：
2014-04-16
影响因子：
4.3
通讯作者：
Rodrigo, J.
中科院分区：
文献类型：
--
作者：
Gharbi, O.;Wuensch, A.;Rodrigo, J.
欧洲李子（Prunus domestica L.） cv ‘Reine Claude Verte’因其出色的感官品质而受到高度赞赏。尽管需求量不断增加，但由于其果实固定不稳定，在许多商业果园中，该品种正在被根除。这种情况促使我们探索特定加入的行为，目的是评估其中一些是否可以更有规律地收割。为此，我们初步评估了在西班牙不同地区收集的24个欧洲“Claude Verte”李子的假定多样性。利用16个李树SSRs进行分子表征。其中8个ssr可转移性来源于以往的属内ssr可转移性研究，另外8个ssr可转移性筛选来源于另外75个李属ssr，其中65个是李属内保守的ssr。通过评价叶片和果实的33个定性性状进行形态鉴定。结果可以通过基因型和表型来区分不同的材料。根据遗传相似性将材料分为6个基因型，根据果实和叶片性状将材料分为7个聚类。在商业栽培品种之间发现了高度的相似性。具有相同基因型谱的部分预测材料表现出形态差异，而具有不同基因型谱的部分预测材料表现出与“莱纳·克劳德·维特”相似的果实性状。分子和形态鉴定的结合使用使得该品种的不同无性系可以被选择作进一步的农艺评价。(C) 2014 Elsevier B.V.版权所有
European plum (Prunus domestica L.) cv 'Reine Claude Verte' is highly appreciated for its excellent organoleptic qualities. Despite its increasing demand, this cultivar is in the process of being eradicated in many commercial orchards because of its generally erratic fruit setting. This situation led us to explore the behaviour of particular accessions with the aim of evaluating if some of them can crop more regularly. For this purpose, we initially evaluated the putative diversity of 24 European plum accessions of 'Reine Claude Verte' collected in different areas of Spain. For the molecular characterization, 16 Prunus SSRs were used. Eight of them were selected from previous works of SSRs transferability within the genus and the other eight were selected from a transferability screening of another 75 Prunus SSRs, in which 65 SSRs were conserved in the species. Morphological characterization was performed by evaluating 33 qualitative characters of leaves and fruits. Results allowed differentiating the accessions by their genotypic profile and their phenotype. The accessions were grouped in six genotypic profiles according to their genetic similarity and in seven clusters according to their fruit and leaf traits. High similarity was found between the 'Reine Claude Verte' commercial cultivars. Some of the prospected accessions sharing the same genotypic profile showed some morphological differences, whereas some accessions with different genotypic profiles presented fruit traits similar to 'Reine Claude Verte'. The combined use of molecular and morphological characterization allowed identifying different clones of the cultivar that were selected for further agronomic evaluation. (C) 2014 Elsevier B.V. All rights reserved.