Generation of Endo-siRNAs in Xenopus laevis Oocytes
Generation of Endo-siRNAs in Xenopus laevis Oocytes
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DOI：
10.1007/978-1-4939-0931-5_3
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
Werner, Andreas
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文献类型：
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作者：
Alnumeir, Sammer;Werner, Andreas
内源性siRNA（endo-siRNA）在C.线虫和果蝇。Endo-siRNA也可以在脊椎动物中发现;然而，它们的生物学不太清楚。它们被认为是由Dicer产生的，并有助于转座子沉默。由于它们的丰度通常较低，并且它们与miRNA和生理RNA翻转产物相似，因此很难研究endo-siRNA。在这里，我们报告了一个系统，卵母细胞从非洲爪蟾，允许从双链RNA前体的内siRNA的生成和分析。
Endogenous siRNAs (endo-siRNAs) are well documented and characterized in C. elegans and Drosophila. Endo-siRNAs can also be found in vertebrates; however, their biology is much less clear. They are thought to be produced by Dicer and to contribute to transposon silencing. Because of their generally low abundance and their similarity with miRNAs and products of physiological RNA turn-over, endo-siRNAs are difficult to investigate. Here, we report a system, oocytes from Xenopus laevis, that allows for the generation and analysis of endo-siRNAs from double-stranded RNA precursors.