Opposite polarity of virus budding and of viral envelope glycoprotein distribution in epithelial cells derived from different tissues.
Opposite polarity of virus budding and of viral envelope glycoprotein distribution in epithelial cells derived from different tissues.
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DOI：
10.1083/jcb.117.3.551
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发表时间：
1992-05
期刊：
影响因子：
--
通讯作者：
Nitsch L
中科院分区：
文献类型：
--
作者：
Zurzolo C;Polistina C;Saini M;Gentile R;Aloj L;Migliaccio G;Bonatti S;Nitsch L
我们比较了四种RNA病毒在Fischer大鼠甲状腺(FRT)细胞和人结肠癌Caco-2细胞中的表面包膜糖蛋白分布和出芽极性。虽然FRT和Caco-2细胞都将流感血凝素和水泡性口炎病毒(VSV)G蛋白分别分类到顶端和基侧膜结构域，但它们在处理两种TOGA病毒Sindbis和Semliki森林病毒(SFV)方面有所不同。常规EM显示，Sindbis病毒和SFV在FRT细胞中顶端萌发，在Caco-2细胞基底部萌发。与这一发现一致的是，免疫电子显微镜和结构域选择性表面生物素化分析表明，SFV的p62/E2包膜糖蛋白在FRT细胞上主要分布在顶端，在Caco-2细胞上分布在底侧。我们得出的结论是，给定的病毒及其包膜糖蛋白可以被传递到来自不同组织的上皮细胞中相反的膜结构域。病毒萌发的极性和病毒包膜糖蛋白分布的组织特异性表明，不同的上皮细胞类型之间的分选机制有很大的差异。
We compared the surface envelope glycoprotein distribution and the budding polarity of four RNA viruses in Fischer rat thyroid (FRT) cells and in CaCo-2 cells derived from a human colon carcinoma. Whereas both FRT and CaCo-2 cells sort similarly influenza hemagglutinin and vesicular stomatitis virus (VSV) G protein, respectively, to apical and basolateral membrane domains, they differ in their handling of two togaviruses, Sindbis and Semliki Forest virus (SFV). By conventional EM Sindbis virus and SFV were shown to bud apically in FRT cells and basolaterally in CaCo-2 cells. Consistent with this finding, the distribution of the p62/E2 envelope glycoprotein of SFV, assayed by immunoelectronmicroscopy and by domain-selective surface biotinylation was predominantly apical on FRT cells and basolateral on CaCo-2 cells. We conclude that a given virus and its envelope glycoprotein can be delivered to opposite membrane domains in epithelial cells derived from different tissues. The tissue specificity in the polarity of virus budding and viral envelope glycoprotein distribution indicate that the sorting machinery varies considerably between different epithelial cell types.