Novel method for mass producing genetically sterile fish from surrogate broodstock via spermatogonial transplantation†
Novel method for mass producing genetically sterile fish from surrogate broodstock via spermatogonial transplantation†
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DOI：
10.1093/biolre/ioy204
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发表时间：
2018-09
影响因子：
3.6
通讯作者：
K. Nagasawa;Mariko Ishida;A. Octavera;Kazunari Kusano;Fumi Kezuka;T. Kitano;Y. Yoshiura;G. Yoshizaki
中科院分区：
文献类型：
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作者：
K. Nagasawa;Mariko Ishida;A. Octavera;Kazunari Kusano;Fumi Kezuka;T. Kitano;Y. Yoshiura;G. Yoshizaki
摘要 需要一种稳定的不育驯化鱼类生产系统，以防止水产养殖逃逸者对当地种群造成遗传污染。本研究的目的是开发一种系统，通过精原细胞移植（SGTP）通过代理亲鱼大规模生产遗传不育的水产养殖种群。我们之前发现，携带卵泡刺激素受体（fshr）基因突变的雌性青鳉鱼会变得不育。在这项研究中，我们证明，接受从性逆转 XX 雄性 (fshr (–/–)) 分离的精原细胞的不育杂交受体雌性恢复了生育能力，并且仅产生供体来源的 fshr (–) X 卵。这些雌性与 fshr (–/–) 性别逆转的 XX 雄性之间的自然交配成功地产生了大量不育的 fshr (–/–) 雌性后代。总之，我们制定了高效大规模生产不育鱼的新策略。该系统可应用于任何可以建立SGTP和生产不育受体的方法的水产养殖物种。摘要句子 通过移植从卵泡刺激激素受体突变体中分离的精原细胞，建立了从代理亲鱼大规模生产遗传不育鱼的新方法
Abstract A stable system for producing sterile domesticated fish is required to prevent genetic contamination to native populations caused by aquaculture escapees. The objective of this study was to develop a system to mass produce stock for aquaculture that is genetically sterile by surrogate broodstock via spermatogonial transplantation (SGTP).We previously discovered that female medaka carrying mutations on the follicle-stimulating hormone receptor (fshr) gene become sterile. In this study, we demonstrated that sterile hybrid recipient females that received spermatogonia isolated from sex-reversed XX males (fshr (–/–)) recovered their fertility and produced only donorderived fshr (–) X eggs. Natural mating between these females and fshr (–/–) sex-reversed XX males successfully produced large numbers of sterile fshr (–/–) female offspring. In conclusion, we established a new strategy for efficient mass production of sterile fish. This system can be applied to any aquaculture species for which SGTP and methods for producing sterile recipients can be established. Summary Sentence Novel method for mass producing genetically sterile fish from surrogate broodstock was established via transplantation of spermatogonia isolated from follicle-stimulating hormone receptor mutants