Studies on primer-dimer formation in polymerase chain reaction (PCR)
Studies on primer-dimer formation in polymerase chain reaction (PCR)
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DOI：
10.1023/a:1008924615839
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发表时间：
1999-10-01
影响因子：
--
通讯作者：
Hsu, JT
中科院分区：
文献类型：
--
作者：
Das, S;Mohapatra, SC;Hsu, JT
根据实验结果，提出了聚合酶链反应（PCR）过程中引物二聚体形成的一种可行机制。该机制导致以Taq DNA聚合酶、两种引物和dNTPs作为起始材料的引物二聚体形成过程的动力学描述。这种引物二聚体的形成对PCR过程的选择性和产率的可能影响进行了讨论。
A viable mechanism for primer-dimer formation in polymerase chain reaction (PCR) process has been proposed based on experimental results. This mechanism results in a kinetic description of the primer-dimer formation process with the Taq DNA polymerase enzyme, the two primers and the dNTPs as the starting materials. The possible impact of this primer-dimer formation on the selectivity and yield of PCR process is discussed.