Immunomodulatory Activity of Docosahexenoic Acid on RAW264.7 Cells Activation through GPR120-Mediated Signaling Pathway
Immunomodulatory Activity of Docosahexenoic Acid on RAW264.7 Cells Activation through GPR120-Mediated Signaling Pathway
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DOI：
10.1021/acs.jafc.7b05894
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发表时间：
2018-01-31
影响因子：
6.1
通讯作者：
Wang, Chunling
中科院分区：
文献类型：
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作者：
Han, Lirong;Yu, Jun;Wang, Chunling
本研究旨在阐明二十二碳六烯酸（DHA）对RAW264.7细胞蛋白表达的免疫调节作用及其分子机制。结果显示，2.4 μ M DHA处理后48 h的RAW 264.7细胞增殖指数约为173.03 ± 7.82%。DHA可通过G蛋白偶联的细胞膜受体GPR 120-C-Raf-丝裂原活化蛋白激酶（MAPK）、核因子κ B（NF-κ B）p65途径激活RAW264.7细胞。另外，2.4 μ M的DHA能显著增加（P < 0.01）诱导型一氧化氮合酶（iNOS）的mRNA和蛋白表达，这与NO释放的结果一致。酶联免疫吸附试验结果显示，二十二碳六烯酸可增强细胞因子IL-1 β、IL-6、IL-10、IL-12、TNF-α、IFN-γ和TGF-β的蛋白表达。这些结果表明DHA对RAW 264. 7细胞的免疫调节机制与通过刺激GPR 120、C-Raf和MAPK向NF-κ B p65通路释放NO和细胞因子有关。
In this study, we elucidated the immunomodulatory activity of docosahexaenoic acid (DHA) on protein expression in RAW264.7 cells and its molecular mechanism. The results showed that the proliferation index of RAW264.7 cells at 48 h was about 173.03 +/- 7.82% after the treatment of 2.4 mu M DHA. DHA could activate RAW264.7 cells by the G-protein coupled cell membrane receptor GPR120-C-Raf-mitogen-activated protein kinases (MAPKs) nuclear factor kappa B (NF-kappa B) p65 pathway. In addition, 2.4 mu M of DHA could significantly increase (P < 0.01) the mRNA and protein expression of inducible nitric oxide synthase (iNOS), which is consistent with the result of the NO release. ELISA results revealed that DHA could enhance the protein expression of cytokines IL-1 beta, IL-6, IL-10, IL-12, TNF-alpha, IFN-gamma, and TGF-beta. These results indicated that the immunomodulatory mechanism of RAW264.7 cells by DHA was associated with the release of NO and cytokines by stimulating the GPR120, C-Raf, and MAPKs to the NF-kappa B p65 pathway.