Enzymatic Production of Biodiesel from Millettia pinnata Seed Oil in Ionic Liquids
Enzymatic Production of Biodiesel from Millettia pinnata Seed Oil in Ionic Liquids
复制标题
DOI：
10.1007/s12155-014-9489-6
复制
发表时间：
2014-12-01
影响因子：
3.6
通讯作者：
Yang, Zhen
中科院分区：
文献类型：
--
作者：
Huang, Ze-Lin;Yang, Tao-Xiang;Yang, Zhen
鸡血藤种子油已被确定为一种有前景的可持续生物柴油生产原料。从淡水和海水栖息地生长的种子中提取的油在其理化性质上没有显着差异。在扩展青霉脂肪酶 (PEL) 和南极假丝酵母脂肪酶 B (Novozym 435) 催化下，在离子液体 (IL) [BMIm][PF6]（1-丁基-3-甲基咪唑鎓六氟磷酸盐）与叔丁醇中研究了油的酶促甲醇分解。在最佳条件下，PEL 在 IL 中诱导的产率比在叔丁醇中高得多，而在 IL 中，PEL 催化的转化率高于 Novozym 435 催化的转化率。通过回收酶 (Novozym 435) 和 IL ([BMIm][PF6]) 以及寻找新酶和新 IL，催化过程得到改进。已经讨论了 IL 对生物柴油生产的影响，并且认为 IL、底物和酶之间的相互作用在控制转化中发挥着重要作用。
Millettia pinnata seed oil has been identified as a promising sustainable feedstock for biodiesel production. Oils extracted from the seeds grown in freshwater and seawater habitats showed no significant differences in their physicochemical properties. The enzymatic methanolysis of the oil has been investigated in the ionic liquid (IL) [BMIm][PF6] (1-butyl-3-methylimidazolium hexafluorophosphate) as compared to tert-butanol, catalyzed by Penicillium expansum lipase (PEL) and Candida antarctica lipase B (Novozym 435). Under optimal conditions, PEL induced much higher yields in the IL than in tert-butanol, while in the IL PEL-catalyzed conversions were higher than those catalyzed by Novozym 435. The catalytic processes were improved by recycling both the enzyme (Novozym 435) and the IL ([BMIm][PF6]) and by searching for new enzymes and new ILs. The impact of ILs on biodiesel production has been discussed, and the interactions between the IL, the substrate, and the enzyme are believed to play an important role in governing the conversion.