Invasion of Oral and Aortic Tissues by Oral Spirochete Treponema denticola in ApoE-/- Mice Causally Links Periodontal Disease and Atherosclerosis
Invasion of Oral and Aortic Tissues by Oral Spirochete Treponema denticola in ApoE-/- Mice Causally Links Periodontal Disease and Atherosclerosis
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DOI：
10.1128/iai.01511-14
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发表时间：
2014-05-01
影响因子：
3.1
通讯作者：
Kesavalu, Lakshmyya
中科院分区：
文献类型：
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作者：
Chukkapalli, Sasanka S.;Rivera, Mercedes F.;Kesavalu, Lakshmyya
齿垢密螺旋体是一种主要存在于龈下的口腔螺旋体，与牙周病密切相关，并在动脉粥样硬化中被检测到。本研究旨在评估慢性口腔T。高脂血症ApoE（-/-）小鼠中的齿垢感染和动脉粥样硬化。ApoE（-/-）小鼠（n = 24）经口感染T. Denticola ATCC 35404，并在12和24周后安乐死。T.在口腔菌斑样品中检测到齿垢菌基因组DNA，表明口腔的定殖。感染引起显着（P = 0.0172）更高的IgG抗体水平和增强骨内缺损比假感染。T.与假感染小鼠相比，感染小鼠的水平牙槽骨吸收水平更高，主动脉斑块面积显著增加（P
Treponema denticola is a predominantly subgingival oral spirochete closely associated with periodontal disease and has been detected in atherosclerosis. This study was designed to evaluate causative links between periodontal disease induced by chronic oral T. denticola infection and atherosclerosis in hyperlipidemic ApoE(-/-) mice. ApoE(-/-) mice (n = 24) were orally infected with T. denticola ATCC 35404 and were euthanized after 12 and 24 weeks. T. denticola genomic DNA was detected in oral plaque samples, indicating colonization of the oral cavity. Infection elicited significantly (P = 0.0172) higher IgG antibody levels and enhanced intrabony defects than sham infection. T. denticola-infected mice had higher levels of horizontal alveolar bone resorption than sham-infected mice and an associated significant increase in aortic plaque area (P