Temperature-Feedback Nanoplatform for NIR-II Penta-Modal Imaging-Guided Synergistic Photothermal Therapy and CAR-NK Immunotherapy of Lung Cancer.
Temperature-Feedback Nanoplatform for NIR-II Penta-Modal Imaging-Guided Synergistic Photothermal Therapy and CAR-NK Immunotherapy of Lung Cancer.
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DOI：
10.1002/smll.202101397
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发表时间：
2021-06
期刊：
影响因子：
13.3
通讯作者：
Mengze Xu;Bin Xue;Yue Wang;Dan Wang;Duyang Gao;Shuo Yang;Qi Zhao;Cang-tao Zhou;S. Ruan
中科院分区：
文献类型：
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作者：
Mengze Xu;Bin Xue;Yue Wang;Dan Wang;Duyang Gao;Shuo Yang;Qi Zhao;Cang-tao Zhou;S. Ruan
在本研究中，为了在进行协同光热治疗(PTT)和肿瘤靶向免疫治疗的同时直观地获取肺癌的全方位结构和功能信息，构建了一个将上转换纳米颗粒(UCNPs)和IR-1048染料引入脂质适体纳米结构(UCILA)的治疗纳米平台。有趣的是，将IR-1048染料嫁接到脂质双层中，可以作为光声成像、光学相干断层扫描血管成像、光热成像和第二近红外(NIR-II)窗口的PTT的治疗试剂。此外，负载在UCILA内部的UCNPs具有优越的发光性能和高的X射线衰减系数，可以作为计算机断层扫描(CT)和热敏上转换发光(UCL)成像的造影剂，实现对肿瘤代谢活动的实时跟踪和温度反馈PTT。此外，在五模式成像的辅助指导下，UCILA在PTT过程中对原位温度变化的准确监测下，以最小的副作用显示了其良好的消融肺肿瘤的能力。同时，协同CAR-NK免疫治疗被专门用于清除PTT后可能残留的肿瘤细胞。因此，UCILA纳米平台被证明是一种多功能治疗剂，可用于五模成像和温度反馈PTT，同时进行肺癌的靶向免疫治疗。
In this study, to visually acquire all-round structural and functional information of lung cancer while performing synergistic photothermal therapy (PTT) and tumor-targeting immunotherapy, a theranostic nanoplatform that introduced upconversion nanoparticles (UCNPs) and IR-1048 dye into the lipid-aptamer nanostructrure (UCILA) is constructed. Interestingly, the IR-1048 dye grafted into the lipid bilayer can serve as the theranostic agent for photoacoustic imaging, optical coherence tomography angiography, photothermal imaging, and PTT in the second near infrared (NIR-II) window. In addition, loaded in the inner part of UCILA, UCNPs possess the superior luminescence property and high X-ray attenuation coefficient, which can act as contrast agents for computed tomography (CT) and thermo-sensitive up-conversion luminescence (UCL) imaging, enabling real-time tracking of metabolic activity of tumor and temperature-feedback PTT. Furthermore, under the complementary guidance of penta-modal imaging and an accurate monitoring of in situ temperature change during PTT, UCILA exhibits its excellent capability for ablating the lung tumor with minimal side effects. Meanwhile, synergistic CAR-NK immunotherapy is carried out specifically to eradicate any possible residual tumor cells after PTT. Therefore, the UCILA nanoplatform is demonstrated as a multifunctional theranostic agent for both penta-modal imaging and temperature-feedback PTT while conducting targeting immunotherapy of lung cancer.