PD-L1-Expressing Dendritic Cells Contribute to Viral Resistance during Acute HSV-1 Infection
PD-L1-Expressing Dendritic Cells Contribute to Viral Resistance during Acute HSV-1 Infection
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DOI：
10.1155/2012/924619
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Carr, Daniel J. J.
中科院分区：
文献类型：
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作者：
Bryant-Hudson, Katie M.;Carr, Daniel J. J.
抑制受体程序性死亡1 （PD-1）及其配体（PD-L1/PD-L2）被认为在慢性病毒感染期间的免疫监视中发挥作用。受体/配体对在急性感染中的作用尚不清楚。为了确定PD-L1和PD-L2在急性眼部单纯疱疹病毒1型（HSV-1）感染中的作用，对感染HSV-1的小鼠给予PD-L1或PD-L2的中和抗体，评估病毒负担和宿主细胞免疫反应。抗PD-L1处理小鼠角膜和三叉神经节（TG）的病毒滴度升高，与cd80表达树突状细胞、PD-L1（+）树突状细胞和引流（下颌）淋巴结（MLN）内hsv -1特异性CD8(+) T细胞数量减少相对应。相比之下，抗pd - l2治疗对病毒复制或MLN群体的变化没有影响。值得注意的是，抗pd - l1处理小鼠的cd11c富集MLN细胞的分析显示功能受损。这些研究表明，在急性HSV-1感染期间，表达pd - l1的树突状细胞对抗病毒防御很重要。
The inhibitory receptor, Programmed Death 1 (PD-1), and its ligands (PD-L1/PD-L2) are thought to play a role in immune surveillance during chronic viral infection. The contribution of the receptor/ligand pair during an acute infection is less understood. To determine the role of PD-L1 and PD-L2 during acute ocular herpes simplex virus type 1 (HSV-1) infection, HSV-1-infected mice administered neutralizing antibody to PD-L1 or PD-L2 were assessed for viral burden and host cellular immune responses. Virus titers were elevated in cornea and trigeminal ganglia (TG) of anti-PD-L1-treated mice which corresponded with a reduced number of CD80-expressing dendritic cells, PD-L1(+) dendritic cells, and HSV-1-specific CD8(+) T cells within the draining (mandibular) lymph node (MLN). In contrast, anti-PD-L2 treatment had no effect on viral replication or changes in the MLN population. Notably, analysis of CD11c-enriched MLN cells from anti-PD-L1-treated mice revealed impaired functional capabilities. These studies indicate PD-L1-expressing dendritic cells are important for antiviral defense during acute HSV-1 infection.