PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice.
PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice.
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PGL 编码叶绿素加氧酶 1，影响叶片衰老并间接影响水稻的产量和品质
DOI：
10.1093/jxb/erv529
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发表时间：
2016-03
影响因子：
6.9
通讯作者：
Zeng D
中科院分区：
文献类型：
--
作者：
Yang Y;Xu J;Huang L;Leng Y;Dai L;Rao Y;Chen L;Wang Y;Tu Z;Hu J;Ren D;Zhang G;Zhu L;Guo L;Qian Q;Zeng D
PGL编码水稻中的CAO 1，其是Chl B合成所必需的，并且影响Chl的合成和降解。PGL还影响叶片衰老，并间接影响籽粒产量和品质。叶绿素（Chl）B是陆生植物、绿色藻类和原绿藻中普遍存在的辅助色素。这种色素由叶绿素a通过叶绿素a加氧酶合成，在适应各种环境中起着关键作用。本研究利用图位克隆的方法，对水稻突变体pale绿色leaf（pgl）进行了鉴定，并分离了PGL基因。PGL编码叶绿素a加氧酶1，主要表达于植物组织中，并在光依赖性叶绿素合成过程中被激活。与野生型植株相比，pgl植株叶绿素含量降低，类囊体超微结构减少、紊乱，光合速率降低，导致籽粒产量和品质下降。此外，pgl在自然和黑暗诱导的条件下都表现出早衰，并且比野生型更严重的叶绿素降解和活性氧积累。而且，pgl对热应激敏感。
PGL encodes CAO1 in rice, which is essential for Chl b synthesis and affects Chl synthesis and degradation. PGL also impacts leaf senescence and indirectly affects grain yield and quality. Chlorophyll (Chl) b is a ubiquitous accessory pigment in land plants, green algae, and prochlorophytes. This pigment is synthesized from Chl a by chlorophyllide a oxygenase and plays a key role in adaptation to various environments. This study characterizes a rice mutant, pale green leaf (pgl), and isolates the gene PGL by using a map-based cloning approach. PGL, encoding chlorophyllide a oxygenase 1, is mainly expressed in the chlorenchyma and activated in the light-dependent Chl synthesis process. Compared with wild-type plants, pgl exhibits a lower Chl content with a reduced and disorderly thylakoid ultrastructure, which decreases the photosynthesis rate and results in reduced grain yield and quality. In addition, pgl exhibits premature senescence in both natural and dark-induced conditions and more severe Chl degradation and reactive oxygen species accumulation than does the wild-type. Moreover, pgl is sensitive to heat stress.