Human platelet aggregation is initiated by peripheral blood mononuclear cells exposed to bacterial lipopolysaccharide in vitro.
Human platelet aggregation is initiated by peripheral blood mononuclear cells exposed to bacterial lipopolysaccharide in vitro.
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人血小板聚集是由体外暴露于细菌脂多糖的外周血单核细胞引发的。
DOI：
10.1172/jci112693
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Monroe,MC
中科院分区：
文献类型：
--
作者：
Schwartz,BS;Monroe,MC
血小板消耗是弥散性血管内凝血的显著特征。我们调查了单核细胞促凝血剂活性(PCA)是否可能在革兰氏阴性败血症相关的血小板消耗中起作用。体外暴露于脂多糖的人单核细胞显示出平行的剂量依赖性的PCA增加和诱导血小板聚集的能力。诱导血小板聚集需要凝血酶的产生依赖于凝血因子VII、X和II以及钙。这与单核细胞组织因子启动凝血酶生成是一致的。用特异性单抗通过间接免疫荧光鉴定单核细胞，证明所有单核细胞都包含在血小板聚集物中。不表达PCA的单核细胞不能诱导血小板聚集，单核细胞周围无血小板聚集。这些数据表明，单核细胞表面诱导表达组织因子可能是内毒素诱导的血小板和纤维蛋白原消耗的重要介质。
Platelet consumption is a prominent feature of disseminated intravascular coagulation. We investigated whether monocyte procoagulant activity (PCA) might play a role in platelet consumption associated with gram-negative septicemia. Human mononuclear cells exposed in vitro to lipopolysaccharide demonstrated parallel dose-dependent increases in PCA and ability to induce platelet aggregation. Induction of platelet aggregation required the generation of thrombin dependent on coagulation Factors VII, X, and II, and calcium. This is consistent with monocyte tissue factor initiating thrombin generation. A specific monoclonal antimonocyte antibody was used to identify monocytes via indirect immunofluorescence, and demonstrated that all monocytes were included in platelet aggregates. Mononuclear cells that did not express PCA did not induce platelet aggregation and monocytes were not surrounded by platelet clumps. These data suggest that monocytes induced to express tissue factor on their surface may be important mediators of endotoxin-induced platelet, as well as fibrinogen, consumption.Images